Regulation of Type 1 Inositol 1,4,5-Trisphosphate–gated Calcium Channels by InsP(3 )and Calcium : Simulation of Single Channel Kinetics Based on Ligand Binding and Electrophysiological Analysis

Cytosolic calcium acts as both a coagonist and an inhibitor of the type 1 inositol 1,4,5-trisphosphate (InsP(3))–gated Ca channel, resulting in a bell-shaped Ca dependence of channel activity (Bezprozvanny, I., J. Watras, and B.E. Ehrlich. 1991. Nature. 351:751–754; Finch, E.A., T.J. Turner, and S.M. Goldin. 1991. Science. 252: 443–446; Iino, M. 1990. J. Gen. Physiol. 95:1103–1122). The ability of Ca to inhibit channel activity, however, varies dramatically depending on InsP(3) concentration (Combettes, L., Z. Hannaert-Merah, J.F. Coquil, C. Rousseau, M. Claret, S. Swillens, and P. Champeil. 1994. J. Biol. Chem. 269:17561–17571; Kaftan, E.J., B.E. Ehrlich, and J. Watras. 1997. J. Gen. Physiol. 110:529–538). In the present report, we have extended the characterization of the effect of cytosolic Ca on both InsP(3) binding and InsP(3)-gated channel kinetics, and incorporated these data into a mathematical model capable of simulating channel kinetics. We found that cytosolic Ca increased the K (d) of InsP(3) binding ∼3.5-fold, but did not influence the maximal number of binding sites. The ability of Ca to decrease InsP(3) binding is consistent with the rightward shift in the bell-shaped Ca dependence of InsP(3)-gated Ca channel activity. High InsP(3) concentrations are able to overcome the Ca-dependent inhibition of channel activity, apparently due to a low affinity InsP(3) binding site (Kaftan, E.J., B.E. Ehrlich, and J. Watras. 1997. J. Gen. Physiol. 110:529–538). Constants from binding analyses and channel activity determinations were used to develop a mathematical model that fits the complex Ca-dependent regulation of the type 1 InsP(3)-gated Ca channel. This model accurately simulated both steady state data (channel open probability and InsP(3) binding) and kinetic data (channel activity and open time distributions), and yielded testable predictions with regard to the regulation of this intracellular Ca channel. Information gained from these analyses, and our current molecular model of this Ca channel, will be important for understanding the basis and regulation of intracellular Ca waves and oscillations in intact cells.