Anomalous Effect of Permeant Ion Concentration on Peak Open Probability of Cardiac Na(+) Channels

Human heart Na(+) channels were expressed transiently in both mammalian cells and Xenopus oocytes, and Na(+) currents measured using 150 mM intracellular Na(+). Decreasing extracellular permeant ion concentration decreases outward Na(+) current at positive voltages while increasing the driving force for the current. This anomalous effect of permeant ion concentration, especially obvious in a mutant (F1485Q) in which fast inactivation is partially abolished, is due to an alteration of open probability. The effect is only observed when a highly permeant cation (Na(+), Li(+), or hydrazinium) is substituted for a relatively impermeant cation (K(+), Rb(+), Cs(+), N -methylglucamine, Tris, choline, or tetramethylammonium). With high concentrations of extracellular permeant cations, the peak open probability of Na(+) channels increases with depolarization and then saturates at positive voltages. By contrast, with low concentrations of permeant ions, the open probability reaches a maximum at approximately 0 mV and then decreases with further depolarization. There is little effect of permeant ion concentration on activation kinetics at depolarized voltages. Furthermore, the lowered open probability caused by a brief depolarization to +60 mV recovers within 5 ms upon repolarization to −140 mV, indicative of a gating process with rapid kinetics. Tail currents at reduced temperatures reveal the rapid onset of this gating process during a large depolarization. A large depolarization may drive a permeant cation out of a site within the extracellular mouth of the pore, reducing the efficiency with which the channel opens.