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Binding of cargo sorting signals to AP-1 enhances its association with ADP ribosylation factor 1–GTP

The adaptor protein AP-1 is the major coat protein involved in the formation of clathrin-coated vesicles at the trans-Golgi network. The prevailing view is that AP-1 recruitment involves coincident binding to multiple low-affinity sites comprising adenosine diphosphate ribosylation factor 1 (Arf-1)–...

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Detalles Bibliográficos
Autores principales: Lee, Intaek, Doray, Balraj, Govero, Jennifer, Kornfeld, Stuart
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2234244/
https://www.ncbi.nlm.nih.gov/pubmed/18250197
http://dx.doi.org/10.1083/jcb.200709037
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author Lee, Intaek
Doray, Balraj
Govero, Jennifer
Kornfeld, Stuart
author_facet Lee, Intaek
Doray, Balraj
Govero, Jennifer
Kornfeld, Stuart
author_sort Lee, Intaek
collection PubMed
description The adaptor protein AP-1 is the major coat protein involved in the formation of clathrin-coated vesicles at the trans-Golgi network. The prevailing view is that AP-1 recruitment involves coincident binding to multiple low-affinity sites comprising adenosine diphosphate ribosylation factor 1 (Arf-1)–guanosine triphosphate (GTP), cargo sorting signals, and phosphoinositides. We now show that binding of cargo signal peptides to AP-1 induces a conformational change in its core domain that greatly enhances its interaction with Arf-1–GTP. In addition, we provide evidence for cross talk between the dileucine and tyrosine binding sites within the AP-1 core domain such that binding of a cargo signal to one site facilitates binding to the other site. The stable association of AP-1 with Arf-1–GTP, which is induced by cargo signals, would serve to provide sufficient time for adaptor polymerization and clathrin recruitment while ensuring the packaging of cargo molecules into the forming transport vesicles.
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spelling pubmed-22342442008-08-11 Binding of cargo sorting signals to AP-1 enhances its association with ADP ribosylation factor 1–GTP Lee, Intaek Doray, Balraj Govero, Jennifer Kornfeld, Stuart J Cell Biol Research Articles The adaptor protein AP-1 is the major coat protein involved in the formation of clathrin-coated vesicles at the trans-Golgi network. The prevailing view is that AP-1 recruitment involves coincident binding to multiple low-affinity sites comprising adenosine diphosphate ribosylation factor 1 (Arf-1)–guanosine triphosphate (GTP), cargo sorting signals, and phosphoinositides. We now show that binding of cargo signal peptides to AP-1 induces a conformational change in its core domain that greatly enhances its interaction with Arf-1–GTP. In addition, we provide evidence for cross talk between the dileucine and tyrosine binding sites within the AP-1 core domain such that binding of a cargo signal to one site facilitates binding to the other site. The stable association of AP-1 with Arf-1–GTP, which is induced by cargo signals, would serve to provide sufficient time for adaptor polymerization and clathrin recruitment while ensuring the packaging of cargo molecules into the forming transport vesicles. The Rockefeller University Press 2008-02-11 /pmc/articles/PMC2234244/ /pubmed/18250197 http://dx.doi.org/10.1083/jcb.200709037 Text en Copyright © 2008, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Lee, Intaek
Doray, Balraj
Govero, Jennifer
Kornfeld, Stuart
Binding of cargo sorting signals to AP-1 enhances its association with ADP ribosylation factor 1–GTP
title Binding of cargo sorting signals to AP-1 enhances its association with ADP ribosylation factor 1–GTP
title_full Binding of cargo sorting signals to AP-1 enhances its association with ADP ribosylation factor 1–GTP
title_fullStr Binding of cargo sorting signals to AP-1 enhances its association with ADP ribosylation factor 1–GTP
title_full_unstemmed Binding of cargo sorting signals to AP-1 enhances its association with ADP ribosylation factor 1–GTP
title_short Binding of cargo sorting signals to AP-1 enhances its association with ADP ribosylation factor 1–GTP
title_sort binding of cargo sorting signals to ap-1 enhances its association with adp ribosylation factor 1–gtp
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2234244/
https://www.ncbi.nlm.nih.gov/pubmed/18250197
http://dx.doi.org/10.1083/jcb.200709037
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