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Human CMV infection induces 5-lipoxygenase expression and leukotriene B(4) production in vascular smooth muscle cells
Leukotrienes (LTs) are powerful proinflammatory lipid mediators that may play a central role in cardiovascular diseases, including arteriosclerosis, myocardial infarction, and stroke. Owing to restricted expression of 5-lipoxygenase (5-LO), the enzyme required for their synthesis, LTs are almost exc...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2234367/ https://www.ncbi.nlm.nih.gov/pubmed/18180307 http://dx.doi.org/10.1084/jem.20070201 |
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author | Qiu, Hong Strååt, Klas Rahbar, Afsar Wan, Min Söderberg-Nauclér, Cecilia Haeggström, Jesper Z. |
author_facet | Qiu, Hong Strååt, Klas Rahbar, Afsar Wan, Min Söderberg-Nauclér, Cecilia Haeggström, Jesper Z. |
author_sort | Qiu, Hong |
collection | PubMed |
description | Leukotrienes (LTs) are powerful proinflammatory lipid mediators that may play a central role in cardiovascular diseases, including arteriosclerosis, myocardial infarction, and stroke. Owing to restricted expression of 5-lipoxygenase (5-LO), the enzyme required for their synthesis, LTs are almost exclusively produced by myeloid cells. Here, we report that human cytomegalovirus (HCMV) infection of human vascular smooth muscle cells (SMCs) increases 5-LO mRNA levels by up to 170-fold in a dose- and time-dependent manner. Infected cells expressed 5-LO protein, as shown by immunohistochemistry, enabling them to synthesize bioactive LTB(4). HCMV-infected vascular SMCs expressing 5-LO protein were readily detected in tissue samples from CMV-infected patients with inflammatory bowel disease or AIDS. Thus, pathogen-induced LT production in HCMV-infected tissues may contribute to local inflammation, consistent with the ability of HCMV to control cellular and immunological functions. HCMV-induced LT biosynthesis in SMCs offers a molecular mechanism to explain HCMV-induced pathogenesis in inflammatory diseases. |
format | Text |
id | pubmed-2234367 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-22343672008-07-21 Human CMV infection induces 5-lipoxygenase expression and leukotriene B(4) production in vascular smooth muscle cells Qiu, Hong Strååt, Klas Rahbar, Afsar Wan, Min Söderberg-Nauclér, Cecilia Haeggström, Jesper Z. J Exp Med Brief Definitive Reports Leukotrienes (LTs) are powerful proinflammatory lipid mediators that may play a central role in cardiovascular diseases, including arteriosclerosis, myocardial infarction, and stroke. Owing to restricted expression of 5-lipoxygenase (5-LO), the enzyme required for their synthesis, LTs are almost exclusively produced by myeloid cells. Here, we report that human cytomegalovirus (HCMV) infection of human vascular smooth muscle cells (SMCs) increases 5-LO mRNA levels by up to 170-fold in a dose- and time-dependent manner. Infected cells expressed 5-LO protein, as shown by immunohistochemistry, enabling them to synthesize bioactive LTB(4). HCMV-infected vascular SMCs expressing 5-LO protein were readily detected in tissue samples from CMV-infected patients with inflammatory bowel disease or AIDS. Thus, pathogen-induced LT production in HCMV-infected tissues may contribute to local inflammation, consistent with the ability of HCMV to control cellular and immunological functions. HCMV-induced LT biosynthesis in SMCs offers a molecular mechanism to explain HCMV-induced pathogenesis in inflammatory diseases. The Rockefeller University Press 2008-01-21 /pmc/articles/PMC2234367/ /pubmed/18180307 http://dx.doi.org/10.1084/jem.20070201 Text en Copyright © 2008, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Brief Definitive Reports Qiu, Hong Strååt, Klas Rahbar, Afsar Wan, Min Söderberg-Nauclér, Cecilia Haeggström, Jesper Z. Human CMV infection induces 5-lipoxygenase expression and leukotriene B(4) production in vascular smooth muscle cells |
title | Human CMV infection induces 5-lipoxygenase expression and leukotriene B(4) production in vascular smooth muscle cells |
title_full | Human CMV infection induces 5-lipoxygenase expression and leukotriene B(4) production in vascular smooth muscle cells |
title_fullStr | Human CMV infection induces 5-lipoxygenase expression and leukotriene B(4) production in vascular smooth muscle cells |
title_full_unstemmed | Human CMV infection induces 5-lipoxygenase expression and leukotriene B(4) production in vascular smooth muscle cells |
title_short | Human CMV infection induces 5-lipoxygenase expression and leukotriene B(4) production in vascular smooth muscle cells |
title_sort | human cmv infection induces 5-lipoxygenase expression and leukotriene b(4) production in vascular smooth muscle cells |
topic | Brief Definitive Reports |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2234367/ https://www.ncbi.nlm.nih.gov/pubmed/18180307 http://dx.doi.org/10.1084/jem.20070201 |
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