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High-throughput screening of monoclonal antibodies against plant cell wall glycans by hierarchical clustering of their carbohydrate microarray binding profiles

Antibody-producing hybridoma cell lines were created following immunisation with a crude extract of cell wall polymers from the plant Arabidopsis thaliana. In order to rapidly screen the specificities of individual monoclonal antibodies (mAbs), their binding to microarrays containing 50 cell wall gl...

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Detalles Bibliográficos
Autores principales: Moller, Isabel, Marcus, Susan E., Haeger, Ash, Verhertbruggen, Yves, Verhoef, Rene, Schols, Henk, Ulvskov, Peter, Mikkelsen, Jørn Dalgaard, Knox, J. Paul, Willats, William
Formato: Texto
Lenguaje:English
Publicado: Springer US 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2234451/
https://www.ncbi.nlm.nih.gov/pubmed/17629746
http://dx.doi.org/10.1007/s10719-007-9059-7