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Relative quantification of mRNA: comparison of methods currently used for real-time PCR data analysis

BACKGROUND: Fluorescent data obtained from real-time PCR must be processed by some method of data analysis to obtain the relative quantity of target mRNA. The method chosen for data analysis can strongly influence results of the quantification. RESULTS: To compare the performance of six techniques w...

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Detalles Bibliográficos
Autores principales: Čikoš, Štefan, Bukovská, Alexandra, Koppel, Juraj
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2235892/
https://www.ncbi.nlm.nih.gov/pubmed/18093344
http://dx.doi.org/10.1186/1471-2199-8-113