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Rapid single step subcloning procedure by combined action of type II and type IIs endonucleases with ligase
BACKGROUND: The subcloning of a DNA fragment from an entry vector into a destination vector is a routinely performed task in molecular biology labs. RESULTS: We here present a novel benchtop procedure to achieve rapid recombination into any destination vector of choice with the sole requirement of a...
| Autores principales: | , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2007
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2241830/ https://www.ncbi.nlm.nih.gov/pubmed/18271995 http://dx.doi.org/10.1186/1754-1611-1-7 |