Intracellular expression profiles measured by real-time PCR tomography in the Xenopus laevis oocyte

Real-time PCR tomography is a novel, quantitative method for measuring localized RNA expression profiles within single cells. We demonstrate its usefulness by dissecting an oocyte from Xenopus laevis into slices along its animal–vegetal axis, extracting its RNA and measuring the levels of 18 selecte...

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Detalles Bibliográficos
Autores principales: Sindelka, Radek, Jonák, Jiri, Hands, Rebecca, Bustin, Stephen A., Kubista, Mikael
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2008
Materias:
Molecular Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2241880/
https://www.ncbi.nlm.nih.gov/pubmed/18039714
http://dx.doi.org/10.1093/nar/gkm1024

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2241880/
https://www.ncbi.nlm.nih.gov/pubmed/18039714
http://dx.doi.org/10.1093/nar/gkm1024

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