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Solution structures of 2 : 1 and 1 : 1 DNA polymerase–DNA complexes probed by ultracentrifugation and small-angle X-ray scattering

We report small-angle X-ray scattering (SAXS) and sedimentation velocity (SV) studies on the enzyme–DNA complexes of rat DNA polymerase β (Pol β) and African swine fever virus DNA polymerase X (ASFV Pol X) with one-nucleotide gapped DNA. The results indicated formation of a 2 : 1 Pol β–DNA complex,...

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Detalles Bibliográficos
Autores principales: Tang, Kuo-Hsiang, Niebuhr, Marc, Aulabaugh, Ann, Tsai, Ming-Daw
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2241917/
https://www.ncbi.nlm.nih.gov/pubmed/18084022
http://dx.doi.org/10.1093/nar/gkm1101