Crystallization of quinoprotein glucose dehydrogenase variants and homologues by microseeding

The soluble quinoprotein glucose dehydrogenase oxidizes glucose, maltose and a variety of other monosaccharides and disaccharides to the corresponding lactones. An efficient microseeding protocol is reported to produce crystals of three variants that display reduced activity towards maltose. Similar...

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Detalles Bibliográficos
Autores principales: Sanchez-Weatherby, Juan, Southall, Stacey, Oubrie, Arthur
Formato: Texto
Lenguaje:English
Publicado: International Union of Crystallography 2006
Materias:
Crystallization Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2243101/
https://www.ncbi.nlm.nih.gov/pubmed/16754970
http://dx.doi.org/10.1107/S1744309106014862
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author Sanchez-Weatherby, Juan
Southall, Stacey
Oubrie, Arthur
author_facet Sanchez-Weatherby, Juan
Southall, Stacey
Oubrie, Arthur
author_sort Sanchez-Weatherby, Juan
collection PubMed
description The soluble quinoprotein glucose dehydrogenase oxidizes glucose, maltose and a variety of other monosaccharides and disaccharides to the corresponding lactones. An efficient microseeding protocol is reported to produce crystals of three variants that display reduced activity towards maltose. Similar cross-seeding protocols to grow crystals of homologues from Escherichia coli and Streptomyces coelicolor are described.
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spelling pubmed-22431012008-03-13 Crystallization of quinoprotein glucose dehydrogenase variants and homologues by microseeding Sanchez-Weatherby, Juan Southall, Stacey Oubrie, Arthur Acta Crystallogr Sect F Struct Biol Cryst Commun Crystallization Communications The soluble quinoprotein glucose dehydrogenase oxidizes glucose, maltose and a variety of other monosaccharides and disaccharides to the corresponding lactones. An efficient microseeding protocol is reported to produce crystals of three variants that display reduced activity towards maltose. Similar cross-seeding protocols to grow crystals of homologues from Escherichia coli and Streptomyces coelicolor are described. International Union of Crystallography 2006-05-05 /pmc/articles/PMC2243101/ /pubmed/16754970 http://dx.doi.org/10.1107/S1744309106014862 Text en © International Union of Crystallography 2006 http://journals.iucr.org/services/termsofuse.html This is an open-access article distributed under the terms described at http://journals.iucr.org/services/termsofuse.html.
spellingShingle Crystallization Communications
Sanchez-Weatherby, Juan
Southall, Stacey
Oubrie, Arthur
Crystallization of quinoprotein glucose dehydrogenase variants and homologues by microseeding
title Crystallization of quinoprotein glucose dehydrogenase variants and homologues by microseeding
title_full Crystallization of quinoprotein glucose dehydrogenase variants and homologues by microseeding
title_fullStr Crystallization of quinoprotein glucose dehydrogenase variants and homologues by microseeding
title_full_unstemmed Crystallization of quinoprotein glucose dehydrogenase variants and homologues by microseeding
title_short Crystallization of quinoprotein glucose dehydrogenase variants and homologues by microseeding
title_sort crystallization of quinoprotein glucose dehydrogenase variants and homologues by microseeding
topic Crystallization Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2243101/
https://www.ncbi.nlm.nih.gov/pubmed/16754970
http://dx.doi.org/10.1107/S1744309106014862
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AT southallstacey crystallizationofquinoproteinglucosedehydrogenasevariantsandhomologuesbymicroseeding
AT oubriearthur crystallizationofquinoproteinglucosedehydrogenasevariantsandhomologuesbymicroseeding

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