The RNA polymerase factory: a robotic in vitro assembly platform for high-throughput production of recombinant protein complexes

The in-depth structure/function analysis of large protein complexes, such as RNA polymerases (RNAPs), requires an experimental platform capable of assembling variants of such enzymes in large numbers in a reproducible manner under defined in vitro conditions. Here we describe a streamlined and integ...

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Detalles Bibliográficos
Autores principales: Nottebaum, Sven, Tan, Lin, Trzaska, Dominika, Carney, Hannah C., Weinzierl, Robert O. J.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2008
Materias:
Nucleic Acid Enzymes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2248744/
https://www.ncbi.nlm.nih.gov/pubmed/18025041
http://dx.doi.org/10.1093/nar/gkm1044

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2248744/
https://www.ncbi.nlm.nih.gov/pubmed/18025041
http://dx.doi.org/10.1093/nar/gkm1044

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