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The RNA polymerase factory: a robotic in vitro assembly platform for high-throughput production of recombinant protein complexes
The in-depth structure/function analysis of large protein complexes, such as RNA polymerases (RNAPs), requires an experimental platform capable of assembling variants of such enzymes in large numbers in a reproducible manner under defined in vitro conditions. Here we describe a streamlined and integ...
| Autores principales: | , , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2008
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2248744/ https://www.ncbi.nlm.nih.gov/pubmed/18025041 http://dx.doi.org/10.1093/nar/gkm1044 |