The translation of recombinant proteins in E. coli can be improved by in silico generating and screening random libraries of a −70/+96 mRNA region with respect to the translation initiation codon

Recombinant protein translation in Escherichia coli may be limited by stable (i.e. low free energy) secondary structures in the mRNA translation initiation region. To circumvent this issue, we have set-up a computer tool called ‘ExEnSo’ (Expression Enhancer Software) that generates a random library...

Descripción completa

Detalles Bibliográficos
Autores principales: Care, S., Bignon, C., Pelissier, M. C., Blanc, E., Canard, B., Coutard, B.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2008
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2248745/
https://www.ncbi.nlm.nih.gov/pubmed/18084029
http://dx.doi.org/10.1093/nar/gkm1097

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2248745/
https://www.ncbi.nlm.nih.gov/pubmed/18084029
http://dx.doi.org/10.1093/nar/gkm1097

Ejemplares similares