From micrograms to picograms: quantitative PCR reduces the material demands of high-throughput sequencing

Current efforts to recover the Neandertal and mammoth genomes by 454 DNA sequencing demonstrate the sensitivity of this technology. However, routine 454 sequencing applications still require microgram quantities of initial material. This is due to a lack of effective methods for quantifying 454 sequ...

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Detalles Bibliográficos
Autores principales: Meyer, Matthias, Briggs, Adrian W., Maricic, Tomislav, Höber, Barbara, Höffner, Barbara, Krause, Johannes, Weihmann, Antje, Pääbo, Svante, Hofreiter, Michael
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2008
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2248761/
https://www.ncbi.nlm.nih.gov/pubmed/18084031
http://dx.doi.org/10.1093/nar/gkm1095

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2248761/
https://www.ncbi.nlm.nih.gov/pubmed/18084031
http://dx.doi.org/10.1093/nar/gkm1095

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