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From micrograms to picograms: quantitative PCR reduces the material demands of high-throughput sequencing
Current efforts to recover the Neandertal and mammoth genomes by 454 DNA sequencing demonstrate the sensitivity of this technology. However, routine 454 sequencing applications still require microgram quantities of initial material. This is due to a lack of effective methods for quantifying 454 sequ...
Autores principales: | Meyer, Matthias, Briggs, Adrian W., Maricic, Tomislav, Höber, Barbara, Höffner, Barbara, Krause, Johannes, Weihmann, Antje, Pääbo, Svante, Hofreiter, Michael |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2248761/ https://www.ncbi.nlm.nih.gov/pubmed/18084031 http://dx.doi.org/10.1093/nar/gkm1095 |
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