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Mapping the Number of Molecules and Brightness in the Laser Scanning Microscope
We describe a technique based on moment-analysis for the measurement of the average number of molecules and brightness in each pixel in fluorescence microscopy images. The average brightness of the particle is obtained from the ratio of the variance to the average intensity at each pixel. To obtain...
| Autores principales: | , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
The Biophysical Society
2008
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2257897/ https://www.ncbi.nlm.nih.gov/pubmed/18096627 http://dx.doi.org/10.1529/biophysj.107.114645 |