Preamplification techniques for real-time RT-PCR analyses of endomyocardial biopsies

BACKGROUND: Due to the limited RNA amounts from endomyocardial biopsies (EMBs) and low expression levels of certain genes, gene expression analyses by conventional real-time RT-PCR are restrained in EMBs. We applied two preamplification techniques, the TaqMan(® )PreAmp Master Mix (T-PreAmp) and a mu...

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Detalles Bibliográficos
Autores principales: Noutsias, Michel, Rohde, Maria, Block, Andrea, Klippert, Katrin, Lettau, Olga, Blunert, Katja, Hummel, Michael, Kühl, Uwe, Lehmkuhl, Hans, Hetzer, Roland, Rauch, Ursula, Poller, Wolfgang, Pauschinger, Matthias, Schultheiss, Heinz P, Volk, Hans D, Kotsch, Katja
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2262094/
https://www.ncbi.nlm.nih.gov/pubmed/18194512
http://dx.doi.org/10.1186/1471-2199-9-3

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2262094/
https://www.ncbi.nlm.nih.gov/pubmed/18194512
http://dx.doi.org/10.1186/1471-2199-9-3

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