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Efficient and reproducible generation of high-expressing, stable human cell lines without need for antibiotic selection

BACKGROUND: Human cell lines are the most innovative choice of host cell for production of biopharmaceuticals since they allow for authentic posttranslational modification of therapeutic proteins. We present a new method for generating high and stable protein expressing cell lines based on human amn...

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Detalles Bibliográficos
Autores principales: Schiedner, Gudrun, Hertel, Sabine, Bialek, Corinna, Kewes, Helmut, Waschütza, Gero, Volpers, Christoph
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2262890/
https://www.ncbi.nlm.nih.gov/pubmed/18269738
http://dx.doi.org/10.1186/1472-6750-8-13