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Mouse TEX15 is essential for DNA double-strand break repair and chromosomal synapsis during male meiosis

During meiosis, homologous chromosomes undergo synapsis and recombination. We identify TEX15 as a novel protein that is required for chromosomal synapsis and meiotic recombination. Loss of TEX15 function in mice causes early meiotic arrest in males but not in females. Specifically, TEX15-deficient s...

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Detalles Bibliográficos
Autores principales: Yang, Fang, Eckardt, Sigrid, Leu, N. Adrian, McLaughlin, K. John, Wang, Peijing Jeremy
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2265566/
https://www.ncbi.nlm.nih.gov/pubmed/18283110
http://dx.doi.org/10.1083/jcb.200709057
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author Yang, Fang
Eckardt, Sigrid
Leu, N. Adrian
McLaughlin, K. John
Wang, Peijing Jeremy
author_facet Yang, Fang
Eckardt, Sigrid
Leu, N. Adrian
McLaughlin, K. John
Wang, Peijing Jeremy
author_sort Yang, Fang
collection PubMed
description During meiosis, homologous chromosomes undergo synapsis and recombination. We identify TEX15 as a novel protein that is required for chromosomal synapsis and meiotic recombination. Loss of TEX15 function in mice causes early meiotic arrest in males but not in females. Specifically, TEX15-deficient spermatocytes exhibit a failure in chromosomal synapsis. In mutant spermatocytes, DNA double-strand breaks (DSBs) are formed, but localization of the recombination proteins RAD51 and DMC1 to meiotic chromosomes is severely impaired. Based on these data, we propose that TEX15 regulates the loading of DNA repair proteins onto sites of DSBs and, thus, its absence causes a failure in meiotic recombination.
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spelling pubmed-22655662008-08-25 Mouse TEX15 is essential for DNA double-strand break repair and chromosomal synapsis during male meiosis Yang, Fang Eckardt, Sigrid Leu, N. Adrian McLaughlin, K. John Wang, Peijing Jeremy J Cell Biol Research Articles During meiosis, homologous chromosomes undergo synapsis and recombination. We identify TEX15 as a novel protein that is required for chromosomal synapsis and meiotic recombination. Loss of TEX15 function in mice causes early meiotic arrest in males but not in females. Specifically, TEX15-deficient spermatocytes exhibit a failure in chromosomal synapsis. In mutant spermatocytes, DNA double-strand breaks (DSBs) are formed, but localization of the recombination proteins RAD51 and DMC1 to meiotic chromosomes is severely impaired. Based on these data, we propose that TEX15 regulates the loading of DNA repair proteins onto sites of DSBs and, thus, its absence causes a failure in meiotic recombination. The Rockefeller University Press 2008-02-25 /pmc/articles/PMC2265566/ /pubmed/18283110 http://dx.doi.org/10.1083/jcb.200709057 Text en Copyright © 2008, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Yang, Fang
Eckardt, Sigrid
Leu, N. Adrian
McLaughlin, K. John
Wang, Peijing Jeremy
Mouse TEX15 is essential for DNA double-strand break repair and chromosomal synapsis during male meiosis
title Mouse TEX15 is essential for DNA double-strand break repair and chromosomal synapsis during male meiosis
title_full Mouse TEX15 is essential for DNA double-strand break repair and chromosomal synapsis during male meiosis
title_fullStr Mouse TEX15 is essential for DNA double-strand break repair and chromosomal synapsis during male meiosis
title_full_unstemmed Mouse TEX15 is essential for DNA double-strand break repair and chromosomal synapsis during male meiosis
title_short Mouse TEX15 is essential for DNA double-strand break repair and chromosomal synapsis during male meiosis
title_sort mouse tex15 is essential for dna double-strand break repair and chromosomal synapsis during male meiosis
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2265566/
https://www.ncbi.nlm.nih.gov/pubmed/18283110
http://dx.doi.org/10.1083/jcb.200709057
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