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Mouse TEX15 is essential for DNA double-strand break repair and chromosomal synapsis during male meiosis
During meiosis, homologous chromosomes undergo synapsis and recombination. We identify TEX15 as a novel protein that is required for chromosomal synapsis and meiotic recombination. Loss of TEX15 function in mice causes early meiotic arrest in males but not in females. Specifically, TEX15-deficient s...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
2008
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2265566/ https://www.ncbi.nlm.nih.gov/pubmed/18283110 http://dx.doi.org/10.1083/jcb.200709057 |
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author | Yang, Fang Eckardt, Sigrid Leu, N. Adrian McLaughlin, K. John Wang, Peijing Jeremy |
author_facet | Yang, Fang Eckardt, Sigrid Leu, N. Adrian McLaughlin, K. John Wang, Peijing Jeremy |
author_sort | Yang, Fang |
collection | PubMed |
description | During meiosis, homologous chromosomes undergo synapsis and recombination. We identify TEX15 as a novel protein that is required for chromosomal synapsis and meiotic recombination. Loss of TEX15 function in mice causes early meiotic arrest in males but not in females. Specifically, TEX15-deficient spermatocytes exhibit a failure in chromosomal synapsis. In mutant spermatocytes, DNA double-strand breaks (DSBs) are formed, but localization of the recombination proteins RAD51 and DMC1 to meiotic chromosomes is severely impaired. Based on these data, we propose that TEX15 regulates the loading of DNA repair proteins onto sites of DSBs and, thus, its absence causes a failure in meiotic recombination. |
format | Text |
id | pubmed-2265566 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-22655662008-08-25 Mouse TEX15 is essential for DNA double-strand break repair and chromosomal synapsis during male meiosis Yang, Fang Eckardt, Sigrid Leu, N. Adrian McLaughlin, K. John Wang, Peijing Jeremy J Cell Biol Research Articles During meiosis, homologous chromosomes undergo synapsis and recombination. We identify TEX15 as a novel protein that is required for chromosomal synapsis and meiotic recombination. Loss of TEX15 function in mice causes early meiotic arrest in males but not in females. Specifically, TEX15-deficient spermatocytes exhibit a failure in chromosomal synapsis. In mutant spermatocytes, DNA double-strand breaks (DSBs) are formed, but localization of the recombination proteins RAD51 and DMC1 to meiotic chromosomes is severely impaired. Based on these data, we propose that TEX15 regulates the loading of DNA repair proteins onto sites of DSBs and, thus, its absence causes a failure in meiotic recombination. The Rockefeller University Press 2008-02-25 /pmc/articles/PMC2265566/ /pubmed/18283110 http://dx.doi.org/10.1083/jcb.200709057 Text en Copyright © 2008, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Research Articles Yang, Fang Eckardt, Sigrid Leu, N. Adrian McLaughlin, K. John Wang, Peijing Jeremy Mouse TEX15 is essential for DNA double-strand break repair and chromosomal synapsis during male meiosis |
title | Mouse TEX15 is essential for DNA double-strand break repair and chromosomal synapsis during male meiosis |
title_full | Mouse TEX15 is essential for DNA double-strand break repair and chromosomal synapsis during male meiosis |
title_fullStr | Mouse TEX15 is essential for DNA double-strand break repair and chromosomal synapsis during male meiosis |
title_full_unstemmed | Mouse TEX15 is essential for DNA double-strand break repair and chromosomal synapsis during male meiosis |
title_short | Mouse TEX15 is essential for DNA double-strand break repair and chromosomal synapsis during male meiosis |
title_sort | mouse tex15 is essential for dna double-strand break repair and chromosomal synapsis during male meiosis |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2265566/ https://www.ncbi.nlm.nih.gov/pubmed/18283110 http://dx.doi.org/10.1083/jcb.200709057 |
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