Cargando…

“Optical Patch-clamping”: Single-channel Recording by Imaging Ca(2+) Flux through Individual Muscle Acetylcholine Receptor Channels

We describe an optical technique using total internal reflection fluorescence (TIRF) microscopy to obtain simultaneous and independent recordings from numerous ion channels via imaging of single-channel Ca(2+) flux. Muscle nicotinic acetylcholine (ACh) receptors made up of αβγδ subunits were express...

Descripción completa

Detalles Bibliográficos
Autores principales: Demuro, Angelo, Parker, Ian
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2266576/
https://www.ncbi.nlm.nih.gov/pubmed/16103278
http://dx.doi.org/10.1085/jgp.200509331