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“Optical Patch-clamping”: Single-channel Recording by Imaging Ca(2+) Flux through Individual Muscle Acetylcholine Receptor Channels
We describe an optical technique using total internal reflection fluorescence (TIRF) microscopy to obtain simultaneous and independent recordings from numerous ion channels via imaging of single-channel Ca(2+) flux. Muscle nicotinic acetylcholine (ACh) receptors made up of αβγδ subunits were express...
Autores principales: | , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2005
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2266576/ https://www.ncbi.nlm.nih.gov/pubmed/16103278 http://dx.doi.org/10.1085/jgp.200509331 |