Detection of methylation in promoter sequences by melting curve analysis-based semiquantitative real time PCR

BACKGROUND: We present two melting curve analysis (MCA)-based semiquantitative real time PCR techniques to detect the promoter methylation status of genes. The first, MCA-MSP, follows the same principle as standard MSP but it is performed in a real time thermalcycler with results being visualized in...

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Detalles Bibliográficos
Autores principales: Lorente, Aiala, Mueller, Wolf, Urdangarín, Edurne, Lázcoz, Paula, von Deimling, Andreas, Castresana, Javier S
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Technical Advance
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2266933/
https://www.ncbi.nlm.nih.gov/pubmed/18298842
http://dx.doi.org/10.1186/1471-2407-8-61

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2266933/
https://www.ncbi.nlm.nih.gov/pubmed/18298842
http://dx.doi.org/10.1186/1471-2407-8-61

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