Cargando…

Detection of methylation in promoter sequences by melting curve analysis-based semiquantitative real time PCR

BACKGROUND: We present two melting curve analysis (MCA)-based semiquantitative real time PCR techniques to detect the promoter methylation status of genes. The first, MCA-MSP, follows the same principle as standard MSP but it is performed in a real time thermalcycler with results being visualized in...

Descripción completa

Detalles Bibliográficos
Autores principales:	Lorente, Aiala, Mueller, Wolf, Urdangarín, Edurne, Lázcoz, Paula, von Deimling, Andreas, Castresana, Javier S
Formato:	Texto
Lenguaje:	English
Publicado:	BioMed Central 2008
Materias:	Technical Advance
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2266933/ https://www.ncbi.nlm.nih.gov/pubmed/18298842 http://dx.doi.org/10.1186/1471-2407-8-61

Ejemplares similares

Quantitation of DNA methylation by melt curve analysis
por: Smith, Eric, et al.
Publicado: (2009)

COLD-PCR enhanced melting curve analysis improves diagnostic accuracy for KRAS mutations in colorectal carcinoma
por: Pritchard, Colin C, et al.
Publicado: (2010)

Whole-genome amplified DNA from stored dried blood spots is reliable in high resolution melting curve and sequencing analysis
por: Winkel, Bo G, et al.
Publicado: (2011)

Genotyping of a tri-allelic polymorphism by a novel melting curve assay in MTHFD1L: an association study of nonsyndromic Cleft in Ireland
por: Minguzzi, Stefano, et al.
Publicado: (2012)

Rapid detection of pathological mutations and deletions of the haemoglobin beta gene (HBB) by High Resolution Melting (HRM) analysis and Gene Ratio Analysis Copy Enumeration PCR (GRACE-PCR)
por: Turner, Andrew, et al.
Publicado: (2016)

Comparison of nested PCR and real time PCR of Herpesvirus infections of central nervous system in HIV patients
por: Drago, Lorenzo, et al.
Publicado: (2004)

Real-Time PCR for detection of herpes simplex virus without nucleic acid extraction
por: Pandori, Mark W, et al.
Publicado: (2006)

Fast detection of Noroviruses using a real-time PCR assay and automated sample preparation
por: Schmid, Michael, et al.
Publicado: (2004)

Rapid and reliable detection of α-globin copy number variations by quantitative real-time PCR
por: Grimholt, Runa M, et al.
Publicado: (2014)

Comparing survival curves based on medians
por: Chen, Zhongxue, et al.
Publicado: (2016)

Development and evaluation of a real-time one step Reverse-Transcriptase PCR for quantitation of Chandipura Virus
por: Kumar, Satyendra, et al.
Publicado: (2008)

Development of a multiplex methylation-specific PCR as candidate triage test for women with an HPV-positive cervical scrape
por: Snellenberg, Suzanne, et al.
Publicado: (2012)

Usefulness of real time PCR for the differentiation and quantification of 652 and JP2 Actinobacillus actinomycetemcomitans genotypes in dental plaque and saliva
por: Orrù, Germano, et al.
Publicado: (2006)

SNPs and real-time quantitative PCR method for constitutional allelic copy number determination, the VPREB1 marker case
por: Frigerio, Marcello, et al.
Publicado: (2011)

Dynamic RMST curves for survival analysis in clinical trials
por: Liao, Jason J. Z., et al.
Publicado: (2020)

Invasive anisakiasis by the parasite Anisakis pegreffii (Nematoda: Anisakidae): diagnosis by real-time PCR hydrolysis probe system and immunoblotting assay
por: Mattiucci, Simonetta, et al.
Publicado: (2017)

Sample-to-Answer and Routine Real-Time RT-PCR: A Comparison of Different Platforms for SARS-CoV-2 Detection
por: Wen, Donghua, et al.
Publicado: (2021)

Development and validation of a multiplex-PCR assay for X-linked intellectual disability
por: Jorge, Paula, et al.
Publicado: (2013)

Evaluation of a novel real-time PCR test based on the ssrA gene for the identification of group B streptococci in vaginal swabs
por: Wernecke, Martina, et al.
Publicado: (2009)

Simultaneous detection of respiratory syncytial virus and human metapneumovirus by one-step multiplex real-time RT-PCR in patients with respiratory symptoms
por: You, Huey-Ling, et al.
Publicado: (2017)

Thinking outside the curve, part I: modeling birthweight distribution
por: Charnigo, Richard, et al.
Publicado: (2010)

Multivariate permutation test to compare survival curves for matched data
por: Galimberti, Stefania, et al.
Publicado: (2013)

Detection of Norovirus genogroup I and II by multiplex real-time RT- PCR using a 3'-minor groove binder-DNA probe
por: Hoehne, Marina, et al.
Publicado: (2006)

Conversion of a molecular classifier obtained by gene expression profiling into a classifier based on real-time PCR: a prognosis predictor for gliomas
por: Kawarazaki, Satoru, et al.
Publicado: (2010)

Rapid detection of carriers with BRCA1 and BRCA2 mutations using high resolution melting analysis
por: Takano, Elena A, et al.
Publicado: (2008)

Sequence diversity within the HA-1 gene as detected by melting temperature assay without oligonucleotide probes
por: Graziano, Claudio, et al.
Publicado: (2005)

High resolution melting analysis for a rapid identification of heterozygous and homozygous sequence changes in the MUTYH gene
por: Tricarico, Rossella, et al.
Publicado: (2011)

Thinking outside the curve, part II: modeling fetal-infant mortality
por: Charnigo, Richard, et al.
Publicado: (2010)

Novel anterior curved incision combined with MIPO for Pilon fracture treatment
por: Wu, Dankai, et al.
Publicado: (2020)

Human immunodeficiency virus type 1 (HIV-1) proviral DNA load in purified CD4+ cells by LightCycler(® )Real-time PCR
por: Kabamba-Mukadi, Benoît, et al.
Publicado: (2005)

Comparative evaluation of the new FDA approved THxID™-BRAF test with high resolution melting and sanger sequencing
por: Marchant, Julie, et al.
Publicado: (2014)

Promoter methylation analysis of O6-methylguanine-DNA methyltransferase in glioblastoma: detection by locked nucleic acid based quantitative PCR using an imprinted gene (SNURF) as a reference
por: Morandi, Luca, et al.
Publicado: (2010)

Screening of exon 11 of BRCA1 gene using the high resolution melting approach for diagnosis in Moroccan breast cancer patients
por: El Khachibi, Meryam, et al.
Publicado: (2015)

Rapid semi-automated quantitative multiplex tandem PCR (MT-PCR) assays for the differential diagnosis of influenza-like illness
por: Szewczuk, Elektra, et al.
Publicado: (2010)

Enhanced secondary analysis of survival data: reconstructing the data from published Kaplan-Meier survival curves
por: Guyot, Patricia, et al.
Publicado: (2012)

High Resolution Melting analysis as a rapid and efficient method of screening for small mutations in the STK11 gene in patients with Peutz-Jeghers syndrome
por: Borun, Pawel, et al.
Publicado: (2013)

Detection of group a streptococcal pharyngitis by quantitative PCR
por: Dunne, Eileen M, et al.
Publicado: (2013)

Quantitative threefold allele-specific PCR (QuanTAS-PCR) for highly sensitive JAK2 V617F mutant allele detection
por: Zapparoli, Giada V, et al.
Publicado: (2013)

Extensions to decision curve analysis, a novel method for evaluating diagnostic tests, prediction models and molecular markers
por: Vickers, Andrew J, et al.
Publicado: (2008)

BMI-for-age graphs with severe obesity percentile curves: tools for plotting cross-sectional and longitudinal youth BMI data
por: Racette, Susan B., et al.
Publicado: (2017)

Cannot write session to /tmp/vufind_sessions/sess_e6g9235n8jmgalpanqn6de2888