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Aberrant epigenetic changes and gene expression in cloned cattle dying around birth
BACKGROUND: Aberrant reprogramming of donor somatic cell nuclei may result in many severe problems in animal cloning. To assess the extent of abnormal epigenetic modifications and gene expression in clones, we simultaneously examined DNA methylation, histone H4 acetylation and expression of six gene...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2268668/ https://www.ncbi.nlm.nih.gov/pubmed/18261243 http://dx.doi.org/10.1186/1471-213X-8-14 |
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author | Lin, Li Li, Qiang Zhang, Lei Zhao, Dingsheng Dai, Yunping Li, Ning |
author_facet | Lin, Li Li, Qiang Zhang, Lei Zhao, Dingsheng Dai, Yunping Li, Ning |
author_sort | Lin, Li |
collection | PubMed |
description | BACKGROUND: Aberrant reprogramming of donor somatic cell nuclei may result in many severe problems in animal cloning. To assess the extent of abnormal epigenetic modifications and gene expression in clones, we simultaneously examined DNA methylation, histone H4 acetylation and expression of six genes (β-actin, VEGF, oct4, TERT, H19 and Igf2) and a repetitive sequence (art2) in five organs (heart, liver, spleen, lung and kidney) from two cloned cattle groups that had died at different stages. In the ED group (early death, n = 3), the cloned cattle died in the perinatal period. The cattle in the LD group (late death, n = 3) died after the perinatal period. Normally reproduced cattle served as a control group (n = 3). RESULTS: Aberrant DNA methylation, histone H4 acetylation and gene expression were observed in both cloned groups. The ED group showed relatively fewer severe DNA methylation abnormalities (p < 0.05) but more abnormal histone H4 acetylations (p < 0.05) and more abnormal expression (p < 0.05) of the selected genes compared to the LD group. However, our data also suggest no widespread gene expression abnormalities in the organs of the dead clones. CONCLUSION: Deaths of clones may be ascribed to abnormal expression of a very limited number of genes. |
format | Text |
id | pubmed-2268668 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-22686682008-03-18 Aberrant epigenetic changes and gene expression in cloned cattle dying around birth Lin, Li Li, Qiang Zhang, Lei Zhao, Dingsheng Dai, Yunping Li, Ning BMC Dev Biol Research Article BACKGROUND: Aberrant reprogramming of donor somatic cell nuclei may result in many severe problems in animal cloning. To assess the extent of abnormal epigenetic modifications and gene expression in clones, we simultaneously examined DNA methylation, histone H4 acetylation and expression of six genes (β-actin, VEGF, oct4, TERT, H19 and Igf2) and a repetitive sequence (art2) in five organs (heart, liver, spleen, lung and kidney) from two cloned cattle groups that had died at different stages. In the ED group (early death, n = 3), the cloned cattle died in the perinatal period. The cattle in the LD group (late death, n = 3) died after the perinatal period. Normally reproduced cattle served as a control group (n = 3). RESULTS: Aberrant DNA methylation, histone H4 acetylation and gene expression were observed in both cloned groups. The ED group showed relatively fewer severe DNA methylation abnormalities (p < 0.05) but more abnormal histone H4 acetylations (p < 0.05) and more abnormal expression (p < 0.05) of the selected genes compared to the LD group. However, our data also suggest no widespread gene expression abnormalities in the organs of the dead clones. CONCLUSION: Deaths of clones may be ascribed to abnormal expression of a very limited number of genes. BioMed Central 2008-02-11 /pmc/articles/PMC2268668/ /pubmed/18261243 http://dx.doi.org/10.1186/1471-213X-8-14 Text en Copyright © 2008 Lin et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Lin, Li Li, Qiang Zhang, Lei Zhao, Dingsheng Dai, Yunping Li, Ning Aberrant epigenetic changes and gene expression in cloned cattle dying around birth |
title | Aberrant epigenetic changes and gene expression in cloned cattle dying around birth |
title_full | Aberrant epigenetic changes and gene expression in cloned cattle dying around birth |
title_fullStr | Aberrant epigenetic changes and gene expression in cloned cattle dying around birth |
title_full_unstemmed | Aberrant epigenetic changes and gene expression in cloned cattle dying around birth |
title_short | Aberrant epigenetic changes and gene expression in cloned cattle dying around birth |
title_sort | aberrant epigenetic changes and gene expression in cloned cattle dying around birth |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2268668/ https://www.ncbi.nlm.nih.gov/pubmed/18261243 http://dx.doi.org/10.1186/1471-213X-8-14 |
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