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Aberrant epigenetic changes and gene expression in cloned cattle dying around birth

BACKGROUND: Aberrant reprogramming of donor somatic cell nuclei may result in many severe problems in animal cloning. To assess the extent of abnormal epigenetic modifications and gene expression in clones, we simultaneously examined DNA methylation, histone H4 acetylation and expression of six gene...

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Autores principales: Lin, Li, Li, Qiang, Zhang, Lei, Zhao, Dingsheng, Dai, Yunping, Li, Ning
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2268668/
https://www.ncbi.nlm.nih.gov/pubmed/18261243
http://dx.doi.org/10.1186/1471-213X-8-14
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author Lin, Li
Li, Qiang
Zhang, Lei
Zhao, Dingsheng
Dai, Yunping
Li, Ning
author_facet Lin, Li
Li, Qiang
Zhang, Lei
Zhao, Dingsheng
Dai, Yunping
Li, Ning
author_sort Lin, Li
collection PubMed
description BACKGROUND: Aberrant reprogramming of donor somatic cell nuclei may result in many severe problems in animal cloning. To assess the extent of abnormal epigenetic modifications and gene expression in clones, we simultaneously examined DNA methylation, histone H4 acetylation and expression of six genes (β-actin, VEGF, oct4, TERT, H19 and Igf2) and a repetitive sequence (art2) in five organs (heart, liver, spleen, lung and kidney) from two cloned cattle groups that had died at different stages. In the ED group (early death, n = 3), the cloned cattle died in the perinatal period. The cattle in the LD group (late death, n = 3) died after the perinatal period. Normally reproduced cattle served as a control group (n = 3). RESULTS: Aberrant DNA methylation, histone H4 acetylation and gene expression were observed in both cloned groups. The ED group showed relatively fewer severe DNA methylation abnormalities (p < 0.05) but more abnormal histone H4 acetylations (p < 0.05) and more abnormal expression (p < 0.05) of the selected genes compared to the LD group. However, our data also suggest no widespread gene expression abnormalities in the organs of the dead clones. CONCLUSION: Deaths of clones may be ascribed to abnormal expression of a very limited number of genes.
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spelling pubmed-22686682008-03-18 Aberrant epigenetic changes and gene expression in cloned cattle dying around birth Lin, Li Li, Qiang Zhang, Lei Zhao, Dingsheng Dai, Yunping Li, Ning BMC Dev Biol Research Article BACKGROUND: Aberrant reprogramming of donor somatic cell nuclei may result in many severe problems in animal cloning. To assess the extent of abnormal epigenetic modifications and gene expression in clones, we simultaneously examined DNA methylation, histone H4 acetylation and expression of six genes (β-actin, VEGF, oct4, TERT, H19 and Igf2) and a repetitive sequence (art2) in five organs (heart, liver, spleen, lung and kidney) from two cloned cattle groups that had died at different stages. In the ED group (early death, n = 3), the cloned cattle died in the perinatal period. The cattle in the LD group (late death, n = 3) died after the perinatal period. Normally reproduced cattle served as a control group (n = 3). RESULTS: Aberrant DNA methylation, histone H4 acetylation and gene expression were observed in both cloned groups. The ED group showed relatively fewer severe DNA methylation abnormalities (p < 0.05) but more abnormal histone H4 acetylations (p < 0.05) and more abnormal expression (p < 0.05) of the selected genes compared to the LD group. However, our data also suggest no widespread gene expression abnormalities in the organs of the dead clones. CONCLUSION: Deaths of clones may be ascribed to abnormal expression of a very limited number of genes. BioMed Central 2008-02-11 /pmc/articles/PMC2268668/ /pubmed/18261243 http://dx.doi.org/10.1186/1471-213X-8-14 Text en Copyright © 2008 Lin et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Lin, Li
Li, Qiang
Zhang, Lei
Zhao, Dingsheng
Dai, Yunping
Li, Ning
Aberrant epigenetic changes and gene expression in cloned cattle dying around birth
title Aberrant epigenetic changes and gene expression in cloned cattle dying around birth
title_full Aberrant epigenetic changes and gene expression in cloned cattle dying around birth
title_fullStr Aberrant epigenetic changes and gene expression in cloned cattle dying around birth
title_full_unstemmed Aberrant epigenetic changes and gene expression in cloned cattle dying around birth
title_short Aberrant epigenetic changes and gene expression in cloned cattle dying around birth
title_sort aberrant epigenetic changes and gene expression in cloned cattle dying around birth
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2268668/
https://www.ncbi.nlm.nih.gov/pubmed/18261243
http://dx.doi.org/10.1186/1471-213X-8-14
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