A Comparison of Donor-Acceptor Pairs for Genetically Encoded FRET Sensors: Application to the Epac cAMP Sensor as an Example

We recently reported on CFP-Epac-YFP, an Epac-based single polypeptide FRET reporter to resolve cAMP levels in living cells. In this study, we compared and optimized the fluorescent protein donor/acceptor pairs for use in biosensors such as CFP-Epac-YFP. Our strategy was to prepare a wide range of c...

Descripción completa

Detalles Bibliográficos
Autores principales: van der Krogt, Gerard N. M., Ogink, Janneke, Ponsioen, Bas, Jalink, Kees
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2008
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2271053/
https://www.ncbi.nlm.nih.gov/pubmed/18382687
http://dx.doi.org/10.1371/journal.pone.0001916

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2271053/
https://www.ncbi.nlm.nih.gov/pubmed/18382687
http://dx.doi.org/10.1371/journal.pone.0001916

Ejemplares similares