Cell-free cloning of highly expanded CTG repeats by amplification of dimerized expanded repeats

We describe conditions for producing uninterrupted expanded CTG repeats consisting of up to 2000 repeats using ϕ29 DNA polymerase. Previously, generation of such repeats was hindered by CTG repeat instability in plasmid vectors maintained in Escherichia coli and poor in vitro ligation of CTG repeat...

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Detalles Bibliográficos
Autores principales: Osborne, Robert J., Thornton, Charles A.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2008
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2275075/
https://www.ncbi.nlm.nih.gov/pubmed/18263610
http://dx.doi.org/10.1093/nar/gkn025

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2275075/
https://www.ncbi.nlm.nih.gov/pubmed/18263610
http://dx.doi.org/10.1093/nar/gkn025

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