Precision and linearity targets for validation of an IFNγ ELISPOT, cytokine flow cytometry, and tetramer assay using CMV peptides

BACKGROUND: Single-cell assays of immune function are increasingly used to monitor T cell responses in immunotherapy clinical trials. Standardization and validation of such assays are therefore important to interpretation of the clinical trial data. Here we assess the levels of intra-assay, inter-as...

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Detalles Bibliográficos
Autores principales: Maecker, Holden T, Hassler, Jeffrey, Payne, Janice K, Summers, Amanda, Comatas, Karrie, Ghanayem, Manar, Morse, Michael A, Clay, Timothy M, Lyerly, Herbert K, Bhatia, Sonny, Ghanekar, Smita A, Maino, Vernon C, delaRosa, Corazon, Disis, Mary L
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2275721/
https://www.ncbi.nlm.nih.gov/pubmed/18366814
http://dx.doi.org/10.1186/1471-2172-9-9

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2275721/
https://www.ncbi.nlm.nih.gov/pubmed/18366814
http://dx.doi.org/10.1186/1471-2172-9-9

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