Simultaneous fitting of real-time PCR data with efficiency of amplification modeled as Gaussian function of target fluorescence

BACKGROUND: In real-time PCR, it is necessary to consider the efficiency of amplification (EA) of amplicons in order to determine initial target levels properly. EAs can be deduced from standard curves, but these involve extra effort and cost and may yield invalid EAs. Alternatively, EA can be extra...

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Detalles Bibliográficos
Autores principales: Batsch, Anke, Noetel, Andrea, Fork, Christian, Urban, Anita, Lazic, Daliborka, Lucas, Tina, Pietsch, Julia, Lazar, Andreas, Schömig, Edgar, Gründemann, Dirk
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2276494/
https://www.ncbi.nlm.nih.gov/pubmed/18267040
http://dx.doi.org/10.1186/1471-2105-9-95

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2276494/
https://www.ncbi.nlm.nih.gov/pubmed/18267040
http://dx.doi.org/10.1186/1471-2105-9-95

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