Use of the lambda Red recombinase system to rapidly generate mutants in Pseudomonas aeruginosa

BACKGROUND: The Red recombinase system of bacteriophage lambda has been used to inactivate chromosomal genes in various bacteria and fungi. The procedure consists of electroporating a polymerase chain reaction (PCR) fragment that was obtained with a 1- or 3-step PCR protocol and that carries an anti...

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Detalles Bibliográficos
Autores principales: Lesic, Biliana, Rahme, Laurence G
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2287187/
https://www.ncbi.nlm.nih.gov/pubmed/18248677
http://dx.doi.org/10.1186/1471-2199-9-20

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2287187/
https://www.ncbi.nlm.nih.gov/pubmed/18248677
http://dx.doi.org/10.1186/1471-2199-9-20

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