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Use of the lambda Red recombinase system to rapidly generate mutants in Pseudomonas aeruginosa
BACKGROUND: The Red recombinase system of bacteriophage lambda has been used to inactivate chromosomal genes in various bacteria and fungi. The procedure consists of electroporating a polymerase chain reaction (PCR) fragment that was obtained with a 1- or 3-step PCR protocol and that carries an anti...
| Autores principales: | , |
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| Formato: | Texto |
| Lenguaje: | English |
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BioMed Central
2008
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2287187/ https://www.ncbi.nlm.nih.gov/pubmed/18248677 http://dx.doi.org/10.1186/1471-2199-9-20 |