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Reversed-phase liquid chromatography coupled on-line to estrogen receptor bioaffinity detection based on fluorescence polarization

We describe the development and validation of a high-resolution screening (HRS) platform which couples gradient reversed-phase high-performance liquid chromatography (RP-HPLC) on-line to estrogen receptor α (ERα) affinity detection using fluorescence polarization (FP). FP, which allows detection at...

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Detalles Bibliográficos
Autores principales: Reinen, Jelle, Kool, Jeroen, Vermeulen, Nico P. E.
Formato: Texto
Lenguaje:English
Publicado: Springer-Verlag 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2287205/
https://www.ncbi.nlm.nih.gov/pubmed/18236033
http://dx.doi.org/10.1007/s00216-008-1833-2