The T7-Primer Is a Source of Experimental Bias and Introduces Variability between Microarray Platforms

Eberwine(-like) amplification of mRNA adds distinct 6–10 bp nucleotide stretches to the 5′ end of amplified RNA transcripts. Analysis of over six thousand microarrays reveals that probes containing motifs complementary to these stretches are associated with aberrantly high signals up to a hundred fo...

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Detalles Bibliográficos
Autores principales: Kerkhoven, Ron M., Sie, Daoud, Nieuwland, Marja, Heimerikx, Mike, De Ronde, Jorma, Brugman, Wim, Velds, Arno
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2008
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2292241/
https://www.ncbi.nlm.nih.gov/pubmed/18431470
http://dx.doi.org/10.1371/journal.pone.0001980

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2292241/
https://www.ncbi.nlm.nih.gov/pubmed/18431470
http://dx.doi.org/10.1371/journal.pone.0001980

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