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The T7-Primer Is a Source of Experimental Bias and Introduces Variability between Microarray Platforms
Eberwine(-like) amplification of mRNA adds distinct 6–10 bp nucleotide stretches to the 5′ end of amplified RNA transcripts. Analysis of over six thousand microarrays reveals that probes containing motifs complementary to these stretches are associated with aberrantly high signals up to a hundred fo...
| Autores principales: | , , , , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2008
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2292241/ https://www.ncbi.nlm.nih.gov/pubmed/18431470 http://dx.doi.org/10.1371/journal.pone.0001980 |