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Improved determination of plasmid copy number using quantitative real-time PCR for monitoring fermentation processes
BACKGROUND: Recombinant protein production in Escherichia coli cells is a complex process, where among other parameters, plasmid copy number, structural and segregational stability of plasmid have an important impact on the success of productivity. It was recognised that a method for accurate and ra...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2311272/ https://www.ncbi.nlm.nih.gov/pubmed/18328094 http://dx.doi.org/10.1186/1475-2859-7-6 |