Improved determination of plasmid copy number using quantitative real-time PCR for monitoring fermentation processes

BACKGROUND: Recombinant protein production in Escherichia coli cells is a complex process, where among other parameters, plasmid copy number, structural and segregational stability of plasmid have an important impact on the success of productivity. It was recognised that a method for accurate and ra...

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Detalles Bibliográficos
Autores principales: Škulj, Mihaela, Okršlar, Veronika, Jalen, Špela, Jevševar, Simona, Slanc, Petra, Štrukelj, Borut, Menart, Viktor
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2311272/
https://www.ncbi.nlm.nih.gov/pubmed/18328094
http://dx.doi.org/10.1186/1475-2859-7-6

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2311272/
https://www.ncbi.nlm.nih.gov/pubmed/18328094
http://dx.doi.org/10.1186/1475-2859-7-6

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