Improved production of human type II procollagen in the yeast Pichia pastoris in shake flasks by a wireless-controlled fed-batch system

BACKGROUND: Here we describe a new technical solution for optimization of Pichia pastoris shake flask cultures with the example of production of stable human type II collagen. Production of recombinant proteins in P. pastoris is usually performed by controlling gene expression with the strong AOX1 p...

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Detalles Bibliográficos
Autores principales: Ruottinen, Maria, Bollok, Monika, Kögler, Martin, Neubauer, Antje, Krause, Mirja, Hämäläinen, Eija-Riitta, Myllyharju, Johanna, Vasala, Antti, Neubauer, Peter
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2315644/
https://www.ncbi.nlm.nih.gov/pubmed/18371201
http://dx.doi.org/10.1186/1472-6750-8-33

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2315644/
https://www.ncbi.nlm.nih.gov/pubmed/18371201
http://dx.doi.org/10.1186/1472-6750-8-33

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