Retrieval of entire genes from environmental DNA by inverse PCR with pre-amplification of target genes using primers containing locked nucleic acids

We had been unsuccessful to amplify desired nucleotide sequences from various environmental DNA samples by using the inverse polymerase chain reaction (IPCR) technique, most probably because the copy numbers of target DNA sequences had been quite low. To enrich the target DNA sequences prior to IPCR...

Descripción completa

Detalles Bibliográficos
Autores principales: Yamada, Kazutaka, Terahara, Takeshi, Kurata, Shinya, Yokomaku, Toyokazu, Tsuneda, Satoshi, Harayama, Shigeaki
Formato: Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2008
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2327201/
https://www.ncbi.nlm.nih.gov/pubmed/18093161
http://dx.doi.org/10.1111/j.1462-2920.2007.01518.x

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2327201/
https://www.ncbi.nlm.nih.gov/pubmed/18093161
http://dx.doi.org/10.1111/j.1462-2920.2007.01518.x

Ejemplares similares