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Crystallization and preliminary X-ray diffraction analysis of ω-amino acid:pyruvate transaminase from Chromobacterium violaceum

The enzyme ω-transaminase catalyses the conversion of chiral ω-amines to ketones. The recombinant enzyme from Chromobacterium violaceum has been purified to homogeneity. The enzyme was crystallized from PEG 4000 using the microbatch method. Data were collected to 1.7 Å resolution from a crystal belo...

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Detalles Bibliográficos
Autores principales: Sayer, Christopher, Isupov, Michail N., Littlechild, Jennifer A.
Formato: Texto
Lenguaje:English
Publicado: International Union of Crystallography 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2330129/
https://www.ncbi.nlm.nih.gov/pubmed/17277454
http://dx.doi.org/10.1107/S1744309107000863