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Molecular cloning, expression, purification and crystallographic analysis of PRRSV 3CL protease

3CL protease, a viral chymotrypsin-like proteolytic enzyme, plays a pivotal role in the transcription and replication machinery of many RNA viruses, including porcine reproductive and respiratory syndrome virus (PRRSV). In this study, the full-length 3CL protease from PRRSV was cloned and overexpres...

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Detalles Bibliográficos
Autores principales: Tian, Xinsheng, Feng, Youjun, Zhao, Tiezhu, Peng, Hao, Yan, Jinghua, Qi, Jianxun, Jiang, Fan, Tian, Kegong, Gao, Feng
Formato: Texto
Lenguaje:English
Publicado: International Union of Crystallography 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2335157/
https://www.ncbi.nlm.nih.gov/pubmed/17671377
http://dx.doi.org/10.1107/S1744309107033234