Cargando…

Molecular cloning, expression, purification and crystallographic analysis of PRRSV 3CL protease

3CL protease, a viral chymotrypsin-like proteolytic enzyme, plays a pivotal role in the transcription and replication machinery of many RNA viruses, including porcine reproductive and respiratory syndrome virus (PRRSV). In this study, the full-length 3CL protease from PRRSV was cloned and overexpres...

Descripción completa

Detalles Bibliográficos
Autores principales:	Tian, Xinsheng, Feng, Youjun, Zhao, Tiezhu, Peng, Hao, Yan, Jinghua, Qi, Jianxun, Jiang, Fan, Tian, Kegong, Gao, Feng
Formato:	Texto
Lenguaje:	English
Publicado:	International Union of Crystallography 2007
Materias:	Crystallization Communications
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2335157/ https://www.ncbi.nlm.nih.gov/pubmed/17671377 http://dx.doi.org/10.1107/S1744309107033234

Ejemplares similares

Cloning, purification and preliminary crystallographic analysis of the Bacillus subtilis GTPase YphC–GDP complex
por: Xu, Ling, et al.
Publicado: (2006)

Cloning, purification and preliminary crystallographic analysis of a putative pyridoxal kinase from Bacillus subtilis
por: Newman, Joseph A., et al.
Publicado: (2006)

Cloning, purification and preliminary crystallographic analysis of a conserved hypothetical protein, SA0961 (YlaN), from Staphylococcus aureus
por: Xu, Ling, et al.
Publicado: (2006)

Expression, purification and preliminary crystallographic analysis of the Toxoplasma gondii enoyl reductase
por: Muench, Stephen P., et al.
Publicado: (2006)

Purification, crystallization and preliminary X-ray crystallographic studies of Rv3705c from Mycobacterium tuberculosis
por: Lu, Feifei, et al.
Publicado: (2014)

Expression, purification and preliminary crystallographic analysis of oligopeptidase B from Trypanosoma brucei
por: Rea, Dean, et al.
Publicado: (2006)

Preliminary crystallographic analysis of avian infectious bronchitis virus main protease
por: Li, Jun, et al.
Publicado: (2006)

Structure and Cleavage Specificity of the Chymotrypsin-Like Serine Protease (3CLSP/nsp4) of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV)
por: Tian, Xinsheng, et al.
Publicado: (2009)

Purification, crystallization and preliminary crystallographic analysis of deoxyuridine triphosphate nucleotidohydrolase from Arabidopsis thaliana
por: Bajaj, Mamta, et al.
Publicado: (2007)

Purification, crystallization and preliminary crystallographic analysis of avian infectious bronchitis virus nsp3 ADRP domain
por: Wei, Lei, et al.
Publicado: (2008)

Crystallization and preliminary crystallographic study of Feline infectious peritonitis virus main protease in complex with an inhibitor
por: Wang, Jinshan, et al.
Publicado: (2014)

Crystallization and preliminary crystallographic study of Porcine epidemic diarrhea virus main protease in complex with an inhibitor
por: Tan, Yusheng, et al.
Publicado: (2014)

Crystallization and preliminary crystallographic study of human coronavirus NL63 main protease in complex with an inhibitor
por: Wang, Fenghua, et al.
Publicado: (2014)

Purification, crystallization and preliminary crystallographic analysis of soybean mature glycinin A1bB2
por: Prak, Krisna, et al.
Publicado: (2013)

Expression, purification, crystallization and preliminary crystallographic analysis of a putative Clostridium difficile surface protein Cwp19
por: Kirby, Jonathan M., et al.
Publicado: (2011)

Purification, crystallization and preliminary crystallographic characterization of the α2,6-sialyltransferase from Photobacterium sp. JT-ISH-224
por: Okino, Nozomu, et al.
Publicado: (2007)

Expression, purification, crystallization and preliminary crystallographic analysis of BipD, a component of the Burkholderia pseudomallei type III secretion system
por: Roversi, Pietro, et al.
Publicado: (2006)

Expression, purification, crystallization and preliminary crystallographic analysis of MxiH, a subunit of the Shigella flexneri type III secretion system needle
por: Deane, Janet E., et al.
Publicado: (2006)

Cloning, purification and crystallization of MotB, a stator component of the proton-driven bacterial flagellar motor
por: O’Neill, Jenna, et al.
Publicado: (2008)

Cloning, purification and preliminary X-ray analysis of the C-terminal domain of Helicobacter pylori MotB
por: Roujeinikova, Anna
Publicado: (2008)

Preliminary X-ray crystallographic analysis of intercellular adhesion molecule-1()
por: Kolatkar, Prasanna R., et al.
Publicado: (1992)

Preliminary crystallographic characterization of PrnB, the second enzyme in the pyrrolnitrin biosynthetic pathway
por: De Laurentis, Walter, et al.
Publicado: (2006)

Refolding, crystallization and preliminary X-ray crystallographic study of the whole extracellular regions of nectins
por: Narita, Hirotaka, et al.
Publicado: (2011)

Expression, crystallization and preliminary crystallographic study of human coronavirus HKU1 nonstructural protein 9
por: Wang, Wei, et al.
Publicado: (2009)

Protein stability: a crystallographer’s perspective
por: Deller, Marc C., et al.
Publicado: (2016)

Expression, crystallization and preliminary crystallographic study of the C-terminal half of nsp2 from SARS coronavirus
por: Li, Yuanyuan, et al.
Publicado: (2011)

Crystallization and preliminary X-ray crystallographic analysis of YfcM: an important factor for EF-P hydroxylation
por: Kobayashi, Kan, et al.
Publicado: (2014)

Expression, crystallization and preliminary crystallographic study of the functional mutant (N60K) of nonstructural protein 9 from Human coronavirus HKU1
por: Chen, Xia, et al.
Publicado: (2014)

Expression, crystallization and preliminary crystallographic study of mouse hepatitis virus (MHV) nucleocapsid protein C-terminal domain
por: Tong, Xiaohang, et al.
Publicado: (2010)

Overexpression, crystallization and preliminary X-ray crystallographic analysis of the C-terminal cytosolic domain of mouse anoctamin 1
por: Park, Sang Ho, et al.
Publicado: (2011)

Crystallization and preliminary X-ray crystallographic analysis of polyphenol oxidase from Juglans regia (jrPPO1)
por: Zekiri, Florime, et al.
Publicado: (2014)

Crystallization and preliminary X-ray crystallographic analysis of latent isoform PPO4 mushroom (Agaricus bisporus) tyrosinase
por: Mauracher, Stephan Gerhard, et al.
Publicado: (2014)

Crystallization and preliminary crystallographic analysis of the major capsid proteins VP16 and VP17 of bacteriophage P23-77
por: Rissanen, Ilona, et al.
Publicado: (2012)

Crystallization and preliminary X-ray crystallographic analysis of the inhibitory domain of the tomato mosaic virus resistance protein Tm-1
por: Kato, Masahiko, et al.
Publicado: (2013)

Expression, limited proteolysis and preliminary crystallographic analysis of IpaD, a component of the Shigella flexneri type III secretion system
por: Johnson, Steven, et al.
Publicado: (2006)

Preliminary X-ray crystallographic analysis of the secreted chorismate mutase from Mycobacterium tuberculosis: a tricky crystallization problem solved
por: Krengel, Ute, et al.
Publicado: (2006)

Production, crystallization and preliminary crystallographic analysis of an exosite-containing fragment of human von Willebrand factor-cleaving proteinase ADAMTS13
por: Akiyama, Masashi, et al.
Publicado: (2009)

Preliminary crystallographic analysis of l-2-keto-3-deoxyarabonate dehydratase, an enzyme involved in an alternative bacterial pathway of l-arabinose metabolism
por: Shimada, Naoko, et al.
Publicado: (2007)

Crystallization and preliminary crystallographic analysis of the bacterial capsule assembly-regulating tyrosine phosphatases Wzb of Escherichia coli and Cps4B of Streptococcus pneumoniae
por: Huang, Hexian, et al.
Publicado: (2009)

Expression, purification and crystallization of the SARS-CoV macro domain
por: Malet, Hélène, et al.
Publicado: (2006)

Cannot write session to /tmp/vufind_sessions/sess_df7gtapi80u70on0tg1b42io7g