Plucked human hair as a tissue in which to assess pharmacodynamic end points during drug development studies

We have demonstrated the feasibility of detecting and quantifying six cell-cycle-related nuclear markers (Ki67, pRb, p27, phospho-p27 (phosphorylated p27), phospho-pRb (phosphorylated pRb), phospho-HH3 (phosphorylated histone H3)) in plucked human scalp and eyebrow hair. Estimates of the proportion...

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Autores principales: Camidge, D R, Randall, K R, Foster, J R, Sadler, C J, Wright, J A, Soames, A R, Laud, P J, Smith, P D, Hughes, A M
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 2005
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2361775/
https://www.ncbi.nlm.nih.gov/pubmed/15886708
http://dx.doi.org/10.1038/sj.bjc.6602558
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author Camidge, D R
Randall, K R
Foster, J R
Sadler, C J
Wright, J A
Soames, A R
Laud, P J
Smith, P D
Hughes, A M
author_facet Camidge, D R
Randall, K R
Foster, J R
Sadler, C J
Wright, J A
Soames, A R
Laud, P J
Smith, P D
Hughes, A M
author_sort Camidge, D R
collection PubMed
description We have demonstrated the feasibility of detecting and quantifying six cell-cycle-related nuclear markers (Ki67, pRb, p27, phospho-p27 (phosphorylated p27), phospho-pRb (phosphorylated pRb), phospho-HH3 (phosphorylated histone H3)) in plucked human scalp and eyebrow hair. Estimates of the proportion of plucked hairs that are lost or damaged during processing plus the intra- and intersubject variability of each nuclear marker with these techniques are provided to inform sizing decisions for intervention studies with drugs potentially impacting on these markers in the future.
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spelling pubmed-23617752009-09-10 Plucked human hair as a tissue in which to assess pharmacodynamic end points during drug development studies Camidge, D R Randall, K R Foster, J R Sadler, C J Wright, J A Soames, A R Laud, P J Smith, P D Hughes, A M Br J Cancer Short Communication We have demonstrated the feasibility of detecting and quantifying six cell-cycle-related nuclear markers (Ki67, pRb, p27, phospho-p27 (phosphorylated p27), phospho-pRb (phosphorylated pRb), phospho-HH3 (phosphorylated histone H3)) in plucked human scalp and eyebrow hair. Estimates of the proportion of plucked hairs that are lost or damaged during processing plus the intra- and intersubject variability of each nuclear marker with these techniques are provided to inform sizing decisions for intervention studies with drugs potentially impacting on these markers in the future. Nature Publishing Group 2005-05-23 2005-05-10 /pmc/articles/PMC2361775/ /pubmed/15886708 http://dx.doi.org/10.1038/sj.bjc.6602558 Text en Copyright © 2005 Cancer Research UK https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Short Communication
Camidge, D R
Randall, K R
Foster, J R
Sadler, C J
Wright, J A
Soames, A R
Laud, P J
Smith, P D
Hughes, A M
Plucked human hair as a tissue in which to assess pharmacodynamic end points during drug development studies
title Plucked human hair as a tissue in which to assess pharmacodynamic end points during drug development studies
title_full Plucked human hair as a tissue in which to assess pharmacodynamic end points during drug development studies
title_fullStr Plucked human hair as a tissue in which to assess pharmacodynamic end points during drug development studies
title_full_unstemmed Plucked human hair as a tissue in which to assess pharmacodynamic end points during drug development studies
title_short Plucked human hair as a tissue in which to assess pharmacodynamic end points during drug development studies
title_sort plucked human hair as a tissue in which to assess pharmacodynamic end points during drug development studies
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2361775/
https://www.ncbi.nlm.nih.gov/pubmed/15886708
http://dx.doi.org/10.1038/sj.bjc.6602558
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