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Lack of EGF receptor contributes to drug sensitivity of human germline cells

Germline mutations have been associated with generation of various types of tumour. In this study, we investigated genetic alteration of germline tumours that affect the drug sensitivity of cells. Although all germline tumour cells we tested were hypersensitive to DNA-damaging drugs, no significant...

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Autores principales: Park, S-J, Armstrong, S, Kim, C-H, Yu, M, Robertson, K, Kelley, M R, Lee, S-H
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2361860/
https://www.ncbi.nlm.nih.gov/pubmed/15655552
http://dx.doi.org/10.1038/sj.bjc.6602315
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author Park, S-J
Armstrong, S
Kim, C-H
Yu, M
Robertson, K
Kelley, M R
Lee, S-H
author_facet Park, S-J
Armstrong, S
Kim, C-H
Yu, M
Robertson, K
Kelley, M R
Lee, S-H
author_sort Park, S-J
collection PubMed
description Germline mutations have been associated with generation of various types of tumour. In this study, we investigated genetic alteration of germline tumours that affect the drug sensitivity of cells. Although all germline tumour cells we tested were hypersensitive to DNA-damaging drugs, no significant alteration was observed in their DNA repair activity or the expression of DNA repair proteins. In contrast, germline tumours expressed very low level of epidermal growth factor receptor (EGFR) compared to drug-resistant ovarian cancer cells. An immunohistochemical analysis indicated that most of the primary germline tumours we tested expressed very low level of EGFR. In accordance with this, overexpression of EGFR in germline tumour cells showed an increase in drug resistance, suggesting that a lack of EGFR, at least in part, contributes to the drug sensitivity of germline tumours.
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spelling pubmed-23618602009-09-10 Lack of EGF receptor contributes to drug sensitivity of human germline cells Park, S-J Armstrong, S Kim, C-H Yu, M Robertson, K Kelley, M R Lee, S-H Br J Cancer Molecular Diagnostics Germline mutations have been associated with generation of various types of tumour. In this study, we investigated genetic alteration of germline tumours that affect the drug sensitivity of cells. Although all germline tumour cells we tested were hypersensitive to DNA-damaging drugs, no significant alteration was observed in their DNA repair activity or the expression of DNA repair proteins. In contrast, germline tumours expressed very low level of epidermal growth factor receptor (EGFR) compared to drug-resistant ovarian cancer cells. An immunohistochemical analysis indicated that most of the primary germline tumours we tested expressed very low level of EGFR. In accordance with this, overexpression of EGFR in germline tumour cells showed an increase in drug resistance, suggesting that a lack of EGFR, at least in part, contributes to the drug sensitivity of germline tumours. Nature Publishing Group 2005-01-31 2005-01-11 /pmc/articles/PMC2361860/ /pubmed/15655552 http://dx.doi.org/10.1038/sj.bjc.6602315 Text en Copyright © 2005 Cancer Research UK https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Molecular Diagnostics
Park, S-J
Armstrong, S
Kim, C-H
Yu, M
Robertson, K
Kelley, M R
Lee, S-H
Lack of EGF receptor contributes to drug sensitivity of human germline cells
title Lack of EGF receptor contributes to drug sensitivity of human germline cells
title_full Lack of EGF receptor contributes to drug sensitivity of human germline cells
title_fullStr Lack of EGF receptor contributes to drug sensitivity of human germline cells
title_full_unstemmed Lack of EGF receptor contributes to drug sensitivity of human germline cells
title_short Lack of EGF receptor contributes to drug sensitivity of human germline cells
title_sort lack of egf receptor contributes to drug sensitivity of human germline cells
topic Molecular Diagnostics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2361860/
https://www.ncbi.nlm.nih.gov/pubmed/15655552
http://dx.doi.org/10.1038/sj.bjc.6602315
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