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Expression in Escherichia coli, purification, refolding and antifungal activity of an osmotin from Solanum nigrum

BACKGROUND: Heterologous protein expression in microorganisms may contribute to identify and demonstrate antifungal activity of novel proteins. The Solanum nigrum osmotin-like protein (SnOLP) gene encodes a member of pathogenesis-related (PR) proteins, from the PR-5 sub-group, the last comprising se...

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Detalles Bibliográficos
Autores principales: Campos, Magnólia de A, Silva, Marilia S, Magalhães, Cláudio P, Ribeiro, Simone G, Sarto, Rafael PD, Vieira, Eduardo A, Grossi de Sá, Maria F
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2362109/
https://www.ncbi.nlm.nih.gov/pubmed/18334031
http://dx.doi.org/10.1186/1475-2859-7-7