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Identification of tumour-associated and germ line p53 mutations in canine mammary cancer
Mutations of the tumour suppressor p53 gene are found in a number of spontaneous canine cancers and may contribute to increased cytogenetic alterations and tumour formation. Using reverse transcription and DNA amplification, we isolated p53 cDNA from normal and tumour tissue of ten canine mammary ca...
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Formato: | Texto |
Lenguaje: | English |
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Nature Publishing Group
1999
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2362910/ https://www.ncbi.nlm.nih.gov/pubmed/10507764 http://dx.doi.org/10.1038/sj.bjc.6690709 |
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author | Veldhoen, N Watterson, J Brash, M Milner, J |
author_facet | Veldhoen, N Watterson, J Brash, M Milner, J |
author_sort | Veldhoen, N |
collection | PubMed |
description | Mutations of the tumour suppressor p53 gene are found in a number of spontaneous canine cancers and may contribute to increased cytogenetic alterations and tumour formation. Using reverse transcription and DNA amplification, we isolated p53 cDNA from normal and tumour tissue of ten canine mammary cancer patients. DNA sequencing identified p53 mutations in three of the ten patients. These included tumour-associated p53 gene mutations within exons 2 and 5 and a germ line deletion of exons 3 to 7. These results support a role for p53 inactivation in canine mammary tumour formation and breed predisposition to cancer. Such information could prove invaluable in the successful outbreeding of inherited predisposition to cancer in the dog. A putative polymorphism was also identified at codon 69 in exon 4 and we discuss the possibility that similar polymorphisms may be associated with human breast cancer. © 1999 Cancer Research Campaign |
format | Text |
id | pubmed-2362910 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1999 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-23629102009-09-10 Identification of tumour-associated and germ line p53 mutations in canine mammary cancer Veldhoen, N Watterson, J Brash, M Milner, J Br J Cancer Regular Article Mutations of the tumour suppressor p53 gene are found in a number of spontaneous canine cancers and may contribute to increased cytogenetic alterations and tumour formation. Using reverse transcription and DNA amplification, we isolated p53 cDNA from normal and tumour tissue of ten canine mammary cancer patients. DNA sequencing identified p53 mutations in three of the ten patients. These included tumour-associated p53 gene mutations within exons 2 and 5 and a germ line deletion of exons 3 to 7. These results support a role for p53 inactivation in canine mammary tumour formation and breed predisposition to cancer. Such information could prove invaluable in the successful outbreeding of inherited predisposition to cancer in the dog. A putative polymorphism was also identified at codon 69 in exon 4 and we discuss the possibility that similar polymorphisms may be associated with human breast cancer. © 1999 Cancer Research Campaign Nature Publishing Group 1999-10 /pmc/articles/PMC2362910/ /pubmed/10507764 http://dx.doi.org/10.1038/sj.bjc.6690709 Text en Copyright © 1999 Cancer Research Campaign https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Regular Article Veldhoen, N Watterson, J Brash, M Milner, J Identification of tumour-associated and germ line p53 mutations in canine mammary cancer |
title | Identification of tumour-associated and germ line p53 mutations in canine mammary cancer |
title_full | Identification of tumour-associated and germ line p53 mutations in canine mammary cancer |
title_fullStr | Identification of tumour-associated and germ line p53 mutations in canine mammary cancer |
title_full_unstemmed | Identification of tumour-associated and germ line p53 mutations in canine mammary cancer |
title_short | Identification of tumour-associated and germ line p53 mutations in canine mammary cancer |
title_sort | identification of tumour-associated and germ line p53 mutations in canine mammary cancer |
topic | Regular Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2362910/ https://www.ncbi.nlm.nih.gov/pubmed/10507764 http://dx.doi.org/10.1038/sj.bjc.6690709 |
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