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A simplified and reliable HPV testing of archival Papanicolaou-stained cervical smears: application to cervical smears from cancer patients starting with cytologically normal smears

The efficacy of four methods to recover DNA from Papanicolaou (Pap)-stained archival cervical smears for optimal detection of human papillomavirus (HPV) DNA by GP5+/bioGP6+ polymerase chain reaction (PCR) was investigated. Two of the methods were based on proteinase K treatment and two based on trea...

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Detalles Bibliográficos
Autores principales: Jacobs, M V, Zielinski, D, Meijer, C J L M, Pol, R P, Voorhorst, F J, de Schipper, F A, Runsink, A P, Snijders, P J F, Walboomers, J M M
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 2000
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2363381/
https://www.ncbi.nlm.nih.gov/pubmed/10780521
http://dx.doi.org/10.1054/bjoc.1999.1128