Progesterone receptor isoforms A and B in human epithelial ovarian carcinoma: immunohistochemical and RT-PCR studies

Human epithelial ovarian carcinoma is well-known as a sex steroid-dependent neoplasm, but the possible biological significance of progesterone receptor (PR) in this cancer remains controversial. Recently, two isoforms of human PR, PRA and PRB, have been characterized and different functional charact...

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Autores principales: Akahira, J, Inoue, T, Suzuki, T, Ito, K, Konno, R, Sato, S, Moriya, T, Okamura, K, Yajima, A, Sasano, H
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 2000
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2363436/
https://www.ncbi.nlm.nih.gov/pubmed/11076658
http://dx.doi.org/10.1054/bjoc.2000.1463
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author Akahira, J
Inoue, T
Suzuki, T
Ito, K
Konno, R
Sato, S
Moriya, T
Okamura, K
Yajima, A
Sasano, H
author_facet Akahira, J
Inoue, T
Suzuki, T
Ito, K
Konno, R
Sato, S
Moriya, T
Okamura, K
Yajima, A
Sasano, H
author_sort Akahira, J
collection PubMed
description Human epithelial ovarian carcinoma is well-known as a sex steroid-dependent neoplasm, but the possible biological significance of progesterone receptor (PR) in this cancer remains controversial. Recently, two isoforms of human PR, PRA and PRB, have been characterized and different functional characteristics have been reported for these two isoforms. We therefore examined immunohistochemistry (107 cases) and reverse transcription-polymerase chain reaction (RT-PCR) (16 cases) for PRA, PRB, and oestrogen receptor-a (ER-a). Labeling indices (LI) for PRA and PRB were 2.4 and 43.6, respectively, and the difference was statistically significant. PRB LI, but not PRA LI, as well as performance status, stage, and residual tumour turned out to be independent prognostic factors following multivariate analysis. There was also a significant correlation between ER-a LI and PRB LI (r = 0.595, P < 0.0001), suggestive of a possible interaction between these two receptors. RT-PCR also detected the expression of PR isoform transcripts in the same pattern as was observed with immunohistochemistry. Results of these studies indicate that PRA and PRB both mediate distinct pathways of progesterone action in ovarian carcinoma. Moreover, it is important to examine PRB LI as a prognostic factor in the cases of human epithelial ovarian carcinoma. © 2000 Cancer Research Campaign http://www.bjcancer.com
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spelling pubmed-23634362009-09-10 Progesterone receptor isoforms A and B in human epithelial ovarian carcinoma: immunohistochemical and RT-PCR studies Akahira, J Inoue, T Suzuki, T Ito, K Konno, R Sato, S Moriya, T Okamura, K Yajima, A Sasano, H Br J Cancer Regular Article Human epithelial ovarian carcinoma is well-known as a sex steroid-dependent neoplasm, but the possible biological significance of progesterone receptor (PR) in this cancer remains controversial. Recently, two isoforms of human PR, PRA and PRB, have been characterized and different functional characteristics have been reported for these two isoforms. We therefore examined immunohistochemistry (107 cases) and reverse transcription-polymerase chain reaction (RT-PCR) (16 cases) for PRA, PRB, and oestrogen receptor-a (ER-a). Labeling indices (LI) for PRA and PRB were 2.4 and 43.6, respectively, and the difference was statistically significant. PRB LI, but not PRA LI, as well as performance status, stage, and residual tumour turned out to be independent prognostic factors following multivariate analysis. There was also a significant correlation between ER-a LI and PRB LI (r = 0.595, P < 0.0001), suggestive of a possible interaction between these two receptors. RT-PCR also detected the expression of PR isoform transcripts in the same pattern as was observed with immunohistochemistry. Results of these studies indicate that PRA and PRB both mediate distinct pathways of progesterone action in ovarian carcinoma. Moreover, it is important to examine PRB LI as a prognostic factor in the cases of human epithelial ovarian carcinoma. © 2000 Cancer Research Campaign http://www.bjcancer.com Nature Publishing Group 2000-12 2000-11-22 /pmc/articles/PMC2363436/ /pubmed/11076658 http://dx.doi.org/10.1054/bjoc.2000.1463 Text en Copyright © 2000 Cancer Research Campaign https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Regular Article
Akahira, J
Inoue, T
Suzuki, T
Ito, K
Konno, R
Sato, S
Moriya, T
Okamura, K
Yajima, A
Sasano, H
Progesterone receptor isoforms A and B in human epithelial ovarian carcinoma: immunohistochemical and RT-PCR studies
title Progesterone receptor isoforms A and B in human epithelial ovarian carcinoma: immunohistochemical and RT-PCR studies
title_full Progesterone receptor isoforms A and B in human epithelial ovarian carcinoma: immunohistochemical and RT-PCR studies
title_fullStr Progesterone receptor isoforms A and B in human epithelial ovarian carcinoma: immunohistochemical and RT-PCR studies
title_full_unstemmed Progesterone receptor isoforms A and B in human epithelial ovarian carcinoma: immunohistochemical and RT-PCR studies
title_short Progesterone receptor isoforms A and B in human epithelial ovarian carcinoma: immunohistochemical and RT-PCR studies
title_sort progesterone receptor isoforms a and b in human epithelial ovarian carcinoma: immunohistochemical and rt-pcr studies
topic Regular Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2363436/
https://www.ncbi.nlm.nih.gov/pubmed/11076658
http://dx.doi.org/10.1054/bjoc.2000.1463
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