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CENP-E combines a slow, processive motor and a flexible coiled coil to produce an essential motile kinetochore tether
The mitotic kinesin centromere protein E (CENP-E) is an essential kinetochore component that directly contributes to the capture and stabilization of spindle microtubules by kinetochores. Although reduction in CENP-E leads to high rates of whole chromosome missegregation, neither its properties as a...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2364708/ https://www.ncbi.nlm.nih.gov/pubmed/18443223 http://dx.doi.org/10.1083/jcb.200802189 |
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author | Kim, Yumi Heuser, John E. Waterman, Clare M. Cleveland, Don W. |
author_facet | Kim, Yumi Heuser, John E. Waterman, Clare M. Cleveland, Don W. |
author_sort | Kim, Yumi |
collection | PubMed |
description | The mitotic kinesin centromere protein E (CENP-E) is an essential kinetochore component that directly contributes to the capture and stabilization of spindle microtubules by kinetochores. Although reduction in CENP-E leads to high rates of whole chromosome missegregation, neither its properties as a microtubule-dependent motor nor how it contributes to the dynamic linkage between kinetochores and microtubules is known. Using single-molecule assays, we demonstrate that CENP-E is a very slow, highly processive motor that maintains microtubule attachment for long periods. Direct visualization of full-length Xenopus laevis CENP-E reveals a highly flexible 230-nm coiled coil separating its kinetochore-binding and motor domains. We also show that full-length CENP-E is a slow plus end–directed motor whose activity is essential for metaphase chromosome alignment. We propose that the highly processive microtubule-dependent motor activity of CENP-E serves to power chromosome congression and provides a flexible, motile tether linking kinetochores to dynamic spindle microtubules. |
format | Text |
id | pubmed-2364708 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-23647082008-11-05 CENP-E combines a slow, processive motor and a flexible coiled coil to produce an essential motile kinetochore tether Kim, Yumi Heuser, John E. Waterman, Clare M. Cleveland, Don W. J Cell Biol Research Articles The mitotic kinesin centromere protein E (CENP-E) is an essential kinetochore component that directly contributes to the capture and stabilization of spindle microtubules by kinetochores. Although reduction in CENP-E leads to high rates of whole chromosome missegregation, neither its properties as a microtubule-dependent motor nor how it contributes to the dynamic linkage between kinetochores and microtubules is known. Using single-molecule assays, we demonstrate that CENP-E is a very slow, highly processive motor that maintains microtubule attachment for long periods. Direct visualization of full-length Xenopus laevis CENP-E reveals a highly flexible 230-nm coiled coil separating its kinetochore-binding and motor domains. We also show that full-length CENP-E is a slow plus end–directed motor whose activity is essential for metaphase chromosome alignment. We propose that the highly processive microtubule-dependent motor activity of CENP-E serves to power chromosome congression and provides a flexible, motile tether linking kinetochores to dynamic spindle microtubules. The Rockefeller University Press 2008-05-05 /pmc/articles/PMC2364708/ /pubmed/18443223 http://dx.doi.org/10.1083/jcb.200802189 Text en © 2008 Kim et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Kim, Yumi Heuser, John E. Waterman, Clare M. Cleveland, Don W. CENP-E combines a slow, processive motor and a flexible coiled coil to produce an essential motile kinetochore tether |
title | CENP-E combines a slow, processive motor and a flexible coiled coil to produce an essential motile kinetochore tether |
title_full | CENP-E combines a slow, processive motor and a flexible coiled coil to produce an essential motile kinetochore tether |
title_fullStr | CENP-E combines a slow, processive motor and a flexible coiled coil to produce an essential motile kinetochore tether |
title_full_unstemmed | CENP-E combines a slow, processive motor and a flexible coiled coil to produce an essential motile kinetochore tether |
title_short | CENP-E combines a slow, processive motor and a flexible coiled coil to produce an essential motile kinetochore tether |
title_sort | cenp-e combines a slow, processive motor and a flexible coiled coil to produce an essential motile kinetochore tether |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2364708/ https://www.ncbi.nlm.nih.gov/pubmed/18443223 http://dx.doi.org/10.1083/jcb.200802189 |
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