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Analysis of cyt0kine gene expression in stimulated T cells of small children by semi-quantitative PCR
Only limited amounts of peripheral blood samples can be obtained from small children. Therefore, a polymerase chain reaction (PCR) aided analysis of cytokine gene expression by PBMC or T cells is a valuable tool. We present a combination of procedures to obtain an accurate estimation of the expressi...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
1995
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2365628/ https://www.ncbi.nlm.nih.gov/pubmed/18475639 http://dx.doi.org/10.1155/S0962935195000329 |