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Analysis of cyt0kine gene expression in stimulated T cells of small children by semi-quantitative PCR

Only limited amounts of peripheral blood samples can be obtained from small children. Therefore, a polymerase chain reaction (PCR) aided analysis of cytokine gene expression by PBMC or T cells is a valuable tool. We present a combination of procedures to obtain an accurate estimation of the expressi...

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Detalles Bibliográficos
Autores principales: Koning, H., Baert, M. R. M., Groot, R. de, Neijens, H. J., Savelkoul, H. F. J.
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 1995
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2365628/
https://www.ncbi.nlm.nih.gov/pubmed/18475639
http://dx.doi.org/10.1155/S0962935195000329