Folding and assembly of large macromolecular complexes monitored by hydrogen-deuterium exchange and mass spectrometry

Recent advances in protein mass spectrometry (MS) have enabled determinations of hydrogen deuterium exchange (HDX) in large macromolecular complexes. HDX-MS became a valuable tool to follow protein folding, assembly and aggregation. The methodology has a wide range of applications in biotechnology r...

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Detalles Bibliográficos
Autores principales: Suchanova, Bohumila, Tuma, Roman
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2365927/
https://www.ncbi.nlm.nih.gov/pubmed/18394161
http://dx.doi.org/10.1186/1475-2859-7-12
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author Suchanova, Bohumila
Tuma, Roman
author_facet Suchanova, Bohumila
Tuma, Roman
author_sort Suchanova, Bohumila
collection PubMed
description Recent advances in protein mass spectrometry (MS) have enabled determinations of hydrogen deuterium exchange (HDX) in large macromolecular complexes. HDX-MS became a valuable tool to follow protein folding, assembly and aggregation. The methodology has a wide range of applications in biotechnology ranging from quality control for over-expressed proteins and their complexes to screening of potential ligands and inhibitors. This review provides an introduction to protein folding and assembly followed by the principles of HDX and MS detection, and concludes with selected examples of applications that might be of interest to the biotechnology community.
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spelling pubmed-23659272008-05-03 Folding and assembly of large macromolecular complexes monitored by hydrogen-deuterium exchange and mass spectrometry Suchanova, Bohumila Tuma, Roman Microb Cell Fact Review Recent advances in protein mass spectrometry (MS) have enabled determinations of hydrogen deuterium exchange (HDX) in large macromolecular complexes. HDX-MS became a valuable tool to follow protein folding, assembly and aggregation. The methodology has a wide range of applications in biotechnology ranging from quality control for over-expressed proteins and their complexes to screening of potential ligands and inhibitors. This review provides an introduction to protein folding and assembly followed by the principles of HDX and MS detection, and concludes with selected examples of applications that might be of interest to the biotechnology community. BioMed Central 2008-04-04 /pmc/articles/PMC2365927/ /pubmed/18394161 http://dx.doi.org/10.1186/1475-2859-7-12 Text en Copyright © 2008 Suchanova and Tuma; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Review
Suchanova, Bohumila
Tuma, Roman
Folding and assembly of large macromolecular complexes monitored by hydrogen-deuterium exchange and mass spectrometry
title Folding and assembly of large macromolecular complexes monitored by hydrogen-deuterium exchange and mass spectrometry
title_full Folding and assembly of large macromolecular complexes monitored by hydrogen-deuterium exchange and mass spectrometry
title_fullStr Folding and assembly of large macromolecular complexes monitored by hydrogen-deuterium exchange and mass spectrometry
title_full_unstemmed Folding and assembly of large macromolecular complexes monitored by hydrogen-deuterium exchange and mass spectrometry
title_short Folding and assembly of large macromolecular complexes monitored by hydrogen-deuterium exchange and mass spectrometry
title_sort folding and assembly of large macromolecular complexes monitored by hydrogen-deuterium exchange and mass spectrometry
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2365927/
https://www.ncbi.nlm.nih.gov/pubmed/18394161
http://dx.doi.org/10.1186/1475-2859-7-12
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