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Post-collection,pre-measurement variables affecting VEGF levels in urine biospecimens

Angiogenesis, the development and recruitment of new blood vessels, plays an important role in tumour growth and metastasis.Vascular endothelial growth factor (VEGF) is an important stimulator of angiogenesis.Circulating and urinary VEGF levels have been suggested as clinically useful predictors of...

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Autores principales: Hayward, Robert M, Kirk, Melissa J, Sproull, Mary, Scott, Tamalee, Smith, Sharon, Cooley-Zgela, Theresa, Crouse, Nancy S, Citrin, Deborah E, Camphausen, Kevin
Formato: Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2367114/
https://www.ncbi.nlm.nih.gov/pubmed/18366457
http://dx.doi.org/10.1111/j.1582-4934.2007.00135.x
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author Hayward, Robert M
Kirk, Melissa J
Sproull, Mary
Scott, Tamalee
Smith, Sharon
Cooley-Zgela, Theresa
Crouse, Nancy S
Citrin, Deborah E
Camphausen, Kevin
author_facet Hayward, Robert M
Kirk, Melissa J
Sproull, Mary
Scott, Tamalee
Smith, Sharon
Cooley-Zgela, Theresa
Crouse, Nancy S
Citrin, Deborah E
Camphausen, Kevin
author_sort Hayward, Robert M
collection PubMed
description Angiogenesis, the development and recruitment of new blood vessels, plays an important role in tumour growth and metastasis.Vascular endothelial growth factor (VEGF) is an important stimulator of angiogenesis.Circulating and urinary VEGF levels have been suggested as clinically useful predictors of tumour behaviour, and investigations into these associations are ongoing.Despite recent interest in measuring VEGF levels in patients, little is known about the factors that influence VEGF levels in biospecimens. To begin to address this question, urine samples were collected from patients with solid tumours undergoing radiotherapy and healthy volunteers.Four factors were examined for their effects on VEGF concentrations as measured by chemiluminescent immunoassay: time from sample collection to freezing, number of specimen freeze–thaw cycles, specimen storage tube type and the inclusion or exclusion of urinary sediment. The results of this study indicate that time to freeze up to 4 hrs, number of freeze–thaw cycles between one and five, and different types of polypropylene tubes did not have statistically significant effects on measured urinary VEGF levels. Urinary sediment had higher VEGF levels than supernatant in five of six samples from healthy patients.It is not clear whether there is an active agent in the sediment causing this increase or if the sediment particles themselves are affecting the accuracy of the assay.Therefore, we recommend centrifuging urine, isolating the supernatant, and freezing the sample in polypropylene microcentrifuge tubes or cryogenic vials within 4 hrs of collection.In addition, we recommend the use of samples within five freeze–thaw cycles.
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spelling pubmed-23671142008-05-05 Post-collection,pre-measurement variables affecting VEGF levels in urine biospecimens Hayward, Robert M Kirk, Melissa J Sproull, Mary Scott, Tamalee Smith, Sharon Cooley-Zgela, Theresa Crouse, Nancy S Citrin, Deborah E Camphausen, Kevin J Cell Mol Med Molecular Diagnosis Angiogenesis, the development and recruitment of new blood vessels, plays an important role in tumour growth and metastasis.Vascular endothelial growth factor (VEGF) is an important stimulator of angiogenesis.Circulating and urinary VEGF levels have been suggested as clinically useful predictors of tumour behaviour, and investigations into these associations are ongoing.Despite recent interest in measuring VEGF levels in patients, little is known about the factors that influence VEGF levels in biospecimens. To begin to address this question, urine samples were collected from patients with solid tumours undergoing radiotherapy and healthy volunteers.Four factors were examined for their effects on VEGF concentrations as measured by chemiluminescent immunoassay: time from sample collection to freezing, number of specimen freeze–thaw cycles, specimen storage tube type and the inclusion or exclusion of urinary sediment. The results of this study indicate that time to freeze up to 4 hrs, number of freeze–thaw cycles between one and five, and different types of polypropylene tubes did not have statistically significant effects on measured urinary VEGF levels. Urinary sediment had higher VEGF levels than supernatant in five of six samples from healthy patients.It is not clear whether there is an active agent in the sediment causing this increase or if the sediment particles themselves are affecting the accuracy of the assay.Therefore, we recommend centrifuging urine, isolating the supernatant, and freezing the sample in polypropylene microcentrifuge tubes or cryogenic vials within 4 hrs of collection.In addition, we recommend the use of samples within five freeze–thaw cycles. Blackwell Publishing Ltd 2008-01 2007-10-23 /pmc/articles/PMC2367114/ /pubmed/18366457 http://dx.doi.org/10.1111/j.1582-4934.2007.00135.x Text en 2008 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd No claim to original US government works
spellingShingle Molecular Diagnosis
Hayward, Robert M
Kirk, Melissa J
Sproull, Mary
Scott, Tamalee
Smith, Sharon
Cooley-Zgela, Theresa
Crouse, Nancy S
Citrin, Deborah E
Camphausen, Kevin
Post-collection,pre-measurement variables affecting VEGF levels in urine biospecimens
title Post-collection,pre-measurement variables affecting VEGF levels in urine biospecimens
title_full Post-collection,pre-measurement variables affecting VEGF levels in urine biospecimens
title_fullStr Post-collection,pre-measurement variables affecting VEGF levels in urine biospecimens
title_full_unstemmed Post-collection,pre-measurement variables affecting VEGF levels in urine biospecimens
title_short Post-collection,pre-measurement variables affecting VEGF levels in urine biospecimens
title_sort post-collection,pre-measurement variables affecting vegf levels in urine biospecimens
topic Molecular Diagnosis
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2367114/
https://www.ncbi.nlm.nih.gov/pubmed/18366457
http://dx.doi.org/10.1111/j.1582-4934.2007.00135.x
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