Requirements for gene silencing mediated by U1 snRNA binding to a target sequence

U1 interference (U1i) is a novel method to block gene expression. U1i requires expression of a 5′-end-mutated U1 snRNA designed to base pair to the 3′-terminal exon of the target gene's pre-mRNA that leads to inhibition of polyadenylation. Here, we show U1i is robust (≥95%) and a 10-nt target l...

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Detalles Bibliográficos
Autores principales: Abad, Xabi, Vera, Maria, Jung, Stephen P., Oswald, Evelyn, Romero, Inés, Amin, Vaibhav, Fortes, Puri, Gunderson, Samuel I.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2008
Materias:
RNA
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2367729/
https://www.ncbi.nlm.nih.gov/pubmed/18299285
http://dx.doi.org/10.1093/nar/gkn068

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2367729/
https://www.ncbi.nlm.nih.gov/pubmed/18299285
http://dx.doi.org/10.1093/nar/gkn068

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